ArticleFunctional & integrative genomics2016
Deep-sequencing transcriptome analysis of field-grown Medicago sativa L. crown buds acclimated to freezing stress.
Article in Functional & integrative genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 40 citations in OpenAlex.
- Unveiling the Cold Acclimation of Alfalfa: Insights into Its Starch-Soluble Sugar Dynamic Transformation.Plants (Basel, Switzerland) · 2025Article
- Identification of Cold Tolerance Transcriptional Regulatory Genes in Seedlings ofInternational journal of molecular sciences · 2024Article
- Exploring the Structure and Substance Metabolism of aInternational journal of molecular sciences · 2024Article
- Physiological characteristics and transcriptomic analyses of alfalfa root crown in wintering.Frontiers in plant science · 2024Article
- Homozygosity mapping identified loci and candidate genes responsible for freezing tolerance in Camelina sativa.The plant genome · 2023Article
- Integrative analysis of transcriptome and metabolome revealed the mechanisms by which flavonoids and phytohormones regulated the adaptation of alfalfa roots to NaCl stress.Frontiers in plant science · 2023Article
- Exogenous application of salicylic acid improves freezing stress tolerance in alfalfa.Frontiers in plant science · 2023Article
- Genome-wide identification and expression analysis ofPlant signaling & behavior · 2022Article
- Genome-Wide Identification ofGenes · 2022Article
- Root rot a silent alfalfa killer in China: Distribution, fungal, and oomycete pathogens, impact of climatic factors and its management.Frontiers in microbiology · 2022Review
- Identification and Characterization of Regulatory Pathways Controlling Dormancy Under Lower Temperature in Alfalfa (Frontiers in plant science · 2022Article
- Low Temperature Stress Tolerance: An Insight Into the Omics Approaches for Legume Crops.Frontiers in plant science · 2022Review
- Comparative Transcriptome Analysis Reveals New Insight of Alfalfa (Frontiers in plant science · 2022Article
- Alfalfa (Medicago sativa L.) MsCML46 gene encoding calmodulin-like protein confers tolerance to abiotic stress in tobacco.Plant cell reports · 2021Article
- Identification of RNA helicases inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- A Review of Unreduced Gametes and Neopolyploids in Alfalfa: How to Fill the Gap between Well-Established Meiotic Mutants and Next-Generation Genomic Resources.Plants (Basel, Switzerland) · 2021Review
- Genome-Wide Association Analysis Coupled With Transcriptome Analysis Reveals Candidate Genes Related to Salt Stress in Alfalfa (Frontiers in plant science · 2021Article
- Biotechnological Perspectives of Omics and Genetic Engineering Methods in Alfalfa.Frontiers in plant science · 2020Review
- High-Throughput Sequencing for Deciphering the Virome of Alfalfa (Frontiers in microbiology · 2020Review
- Identification of emerging viral genomes in transcriptomic datasets of alfalfa (Medicago sativa L.).Virology journal · 2019Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Medicago sativa L. (alfalfa) 'Zhaodong' is an important forage legume that can safely survive in northern China where winter temperatures reach as low as -30 °C. Survival of alfalfa following freezing stress depends on the amount and revival ability of crown buds. In order to investigate the molecular mechanisms of frost tolerance in alfalfa, we used transcriptome sequencing technology and bioinformatics strategies to analyze crown buds of field-grown alfalfa during winter. We statistically identified a total of 5605 differentially expressed genes (DEGs) involved in freezing stress including 1900 upregulated and 3705 downregulated DEGs. We validated 36 candidate DEGs using qPCR to confirm the accuracy of the RNA-seq data. Unlike other recent studies, this study employed alfalfa plants grown in the natural environment. Our results indicate that not only the CBF orthologs but also membrane proteins, hormone signal transduction pathways, and ubiquitin-mediated proteolysis pathways indicate the presence of a special freezing adaptation mechanism in alfalfa. The antioxidant defense system may rapidly confer freezing tolerance to alfalfa. Importantly, biosynthesis of secondary metabolites and phenylalanine metabolism, which is of potential importance in coordinating freezing tolerance with growth and development, were downregulated in subzero temperatures. The adaptive mechanism for frost tolerance is a complex multigenic process that is not well understood. This systematic analysis provided an in-depth view of stress tolerance mechanisms in alfalfa.
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