ArticleScientific reports2016
Acousto-microfluidics for screening of ssDNA aptamer.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- PSA-Responsive Aptamer-Based Switchable Aggregates of Ultrasmall Gold Nanoparticles.Sensors (Basel, Switzerland) · 2025Article
- [Advances in exosome-targeting aptamer-screening techniques].Se pu = Chinese journal of chromatography · 2025Review
- SERS-based CRISPR/Cas12a assays for protein biomarker prostate-specific antigen detection.Analytical and bioanalytical chemistry · 2025Article
- Recent advances in aptamer discovery, modification and improving performance.Biochemistry and biophysics reports · 2024Review
- Nanoarchitectonics-based electrochemical aptasensors for highly efficient exosome detection.Science and technology of advanced materials · 2024Review
- Synergetic collision and space separation in microfluidic chip for efficient affinity-discriminated molecular selection.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Surface acoustic wave-assisted microfluidic isolation of aptamers.Microfluidics and nanofluidics · 2022Article
- The Potential of Aptamer-Mediated Liquid Biopsy for Early Detection of Cancer.International journal of molecular sciences · 2021Review
- Recent Advances in Nanomaterial-Based Aptasensors in Medical Diagnosis and Therapy.Nanomaterials (Basel, Switzerland) · 2021Review
- Flexural wave-based soft attractor walls for trapping microparticles and cells.Lab on a chip · 2021Article
- The Application of Microfluidic Technologies in Aptamer Selection.Frontiers in cell and developmental biology · 2021Review
- Implementation of High-Throughput Sequencing (HTS) in Aptamer Selection Technology.International journal of molecular sciences · 2020Review
- Aptamer Affinity-Bead Mediated Capture and Displacement of Gram-Negative Bacteria Using Acoustophoresis.Micromachines · 2019Article
- Recent Advances in Aptamer Discovery and Applications.Molecules (Basel, Switzerland) · 2019Review
- Microfluidic methods for aptamer selection and characterization.The Analyst · 2017Review
- Recent Advances in SELEX Technology and Aptamer Applications in Biomedicine.International journal of molecular sciences · 2017Review
- Molecular Elucidation of Disease Biomarkers at the Interface of Chemistry and Biology.Journal of the American Chemical Society · 2017Article
- Aptamer Technology: Adjunct Therapy for Malaria.Biomedicines · 2017Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We demonstrate a new screening method for obtaining a prostate-specific antigen (PSA) binding aptamer based on an acoustofluidic separation (acoustophoreis) technique. Since acoustophoresis provides simultaneous washing and separation in a continuous flow mode, we efficiently obtained a PSA binding aptamer that shows high affinity without any additional washing step, which is necessary in other screening methods. In addition, next-generation sequencing (NGS) was applied to accelerate the identification of the screened ssDNA pool, improving the selecting process of the aptamer candidate based on the frequency ranking of the sequences. After the 8(th) round of the acoustophoretic systematic evolution of ligands by exponential enrichment (SELEX) and following sequence analysis with NGS, 7 PSA binding ssDNA aptamer-candidates were obtained and characterized with surface plasmon resonance (SPR) for affinity and specificity. As a result of the new SELEX method with PSA as the model target protein, the best PSA binding aptamer showed specific binding to PSA with a dissociation constant (Kd) of 0.7 nM.
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