ArticleScientific reports2016
Metagenomic discovery of novel enzymes and biosurfactants in a slaughterhouse biofilm microbial community.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Generalizable Direct Protein Sequencing With InstaNexus.Molecular & cellular proteomics : MCP · 2026Article
- Genome-mining revealed biosurfactant production capacity and gene co-occurrence patterns in diverse ecosystems.Microbial cell factories · 2026Article
- Curtobacterium oceanosedimentum-derived biosurfactants as eco-friendly biocontrol agent with broad-spectrum antibacterial and antifungal properties for sustainable applications.World journal of microbiology & biotechnology · 2025Article
- Chemical quantification of N-acyl alanine methyl ester (NAME) production and impact on temporal gene expression patterns in Roseovarius tolerans EL-164.BMC microbiology · 2024Article
- Perspectives of aminoacylases in biocatalytic synthesis of N-acyl-amino acids surfactants.Applied microbiology and biotechnology · 2024Review
- Review
- Screening Strategies for Biosurfactant Discovery.Advances in biochemical engineering/biotechnology · 2022Article
- Potential Use of Microbial Community Genomes in Various Dimensions of Agriculture Productivity and Its Management: A Review.Frontiers in microbiology · 2022Review
- Fungal biosurfactants, from nature to biotechnological product: bioprospection, production and potential applications.Bioprocess and biosystems engineering · 2021Review
- The Biosynthesis and Metabolism of theFrontiers in molecular biosciences · 2021Review
- Tapping the Role of Microbial Biosurfactants in Pesticide Remediation: An Eco-Friendly Approach for Environmental Sustainability.Frontiers in microbiology · 2021Review
- The planctomycete Stieleria maiorica Mal15Communications biology · 2020Article
- Metagenomics-Guided Discovery of Potential Bacterial Metallothionein Genes from the Soil Microbiome That Confer Cu and/or Cd Resistance.Applied and environmental microbiology · 2020Article
- Extreme environments: a source of biosurfactants for biotechnological applications.Extremophiles : life under extreme conditions · 2020Review
- MBSP1: a biosurfactant protein derived from a metagenomic library with activity in oil degradation.Scientific reports · 2020Article
- Agar plate-based screening methods for the identification of polyester hydrolysis by Pseudomonas species.Microbial biotechnology · 2020Article
- Discovery of Novel Biosynthetic Gene Cluster Diversity From a Soil Metagenomic Library.Frontiers in microbiology · 2020Article
- A Straightforward Assay for Screening and Quantification of Biosurfactants in Microbial Culture Supernatants.Frontiers in bioengineering and biotechnology · 2020Article
- Marine Biosurfactants: Biosynthesis, Structural Diversity and Biotechnological Applications.Marine drugs · 2019Review
- Metagenomic Insights Into the Taxonomic and Functional Features ofFrontiers in microbiology · 2019Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA derived from environmental samples is a rich source of novel bioactive molecules. The choice of the habitat to be sampled predefines the properties of the biomolecules to be discovered due to the physiological adaptation of the microbial community to the prevailing environmental conditions. We have constructed a metagenomic library in Escherichia coli DH10b with environmental DNA (eDNA) isolated from the microbial community of a slaughterhouse drain biofilm consisting mainly of species from the family Flavobacteriaceae. By functional screening of this library we have identified several lipases, proteases and two clones (SA343 and SA354) with biosurfactant and hemolytic activities. Sequence analysis of the respective eDNA fragments and subsequent structure homology modelling identified genes encoding putative N-acyl amino acid synthases with a unique two-domain organisation. The produced biosurfactants were identified by NMR spectroscopy as N-acyltyrosines with N-myristoyltyrosine as the predominant species. Critical micelle concentration and reduction of surface tension were similar to those of chemically synthesised N-myristoyltyrosine. Furthermore, we showed that the newly isolated N-acyltyrosines exhibit antibiotic activity against various bacteria. This is the first report describing the successful application of functional high-throughput screening assays for the identification of biosurfactant producing clones within a metagenomic library.
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