Evidence map›Paper›PMID 27271198›Full record

ArticleNature methods2016

Dense transcript profiling in single cells by image correlation decoding.

Ahmet F Coskun, Long Cai

Abstract read
In one paragraph

Article in Nature methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Review
  5. Review
  6. Craniofacial developmental biology in the single-cell era.Development (Cambridge, England) · 2023
    Review
  7. Spatial biology of cancer evolution.Nature reviews. Genetics · 2023
    Review
  8. Review
  9. Review
  10. Review
  11. Review
  12. Review
  13. High-Dimensional Tissue Profiling by Multiplexed Ion Beam Imaging.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  14. Article
  15. Article
  16. Review
  17. Review
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

2 authors.

Ahmet F CoskunDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California, USA.
Long CaiDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California, USA.

Funding

Multiplex RNA imaging in single cells by superresolution microscopy& barcode FISHR01HD075605 · NICHD · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI CAI, LONG, SOMLO, GEORGE NONE · 2012 to 2016
$4.1M
Genomic site binding rules and regulatory factor function in developing T cellsR01HD076915 · NICHD · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI ROTHENBERG, ELLEN V. · 2013 to 2017
$2.5M
NICHD NIH HHS R01 HD075605NICHD NIH HHS R01 HD076915
6 · The paper itself

Abstract

Sequential barcoded fluorescent in situ hybridization (seqFISH) allows large numbers of molecular species to be accurately detected in single cells, but multiplexing is limited by the density of barcoded objects. We present correlation FISH (corrFISH), a method to resolve dense temporal barcodes in sequential hybridization experiments. Using corrFISH, we quantified highly expressed ribosomal protein genes in single cultured cells and mouse thymus sections, revealing cell-type-specific gene expression.

Indexed as

Gene Expression ProfilingAnimalsDietary ProteinsIn Situ Hybridization, FluorescenceMiceNIH 3T3 CellsNucleic Acid HybridizationRibosomal ProteinsRNA, MessengerSingle-Cell AnalysisTranscription, GeneticDietary ProteinsRibosomal ProteinsRNA, Messengersingle cell proteins

Identifiers

PMID27271198
PMCPMC4965285

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.