ArticlePloS one2016
Directed Evolution of FLS2 towards Novel Flagellin Peptide Recognition.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Beyond the Halo: Molecular Mechanisms and Ecological Aspects of the Infection Strategy ofInternational journal of molecular sciences · 2026Review
- Reverse engineering of the pattern recognition receptor FLS2 reveals key design principles of broader recognition spectra against evading flg22 epitopes.Nature plants · 2025Article
- Unlocking expanded flagellin perception through rational receptor engineering.Nature plants · 2025Article
- The resistance awakens: Diversity at the DNA, RNA, and protein levels informs engineering of plant immune receptors from Arabidopsis to crops.The Plant cell · 2025Review
- Plant pattern recognition receptors: from evolutionary insight to engineering.Nature reviews. Genetics · 2025Review
- Directed Evolution of a Plant Immune Receptor for Broad Spectrum Effector Recognition.bioRxiv : the preprint server for biology · 2025Article
- All Roads Lead to Rome: Pathways to Engineering Disease Resistance in Plants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Flagellin, a plant-defense-activating protein identified from Xanthomonas axonopodis pv. Dieffenbachiae invokes defense response in tobacco.BMC microbiology · 2023Article
- An MKP-MAPK protein phosphorylation cascade controls vascular immunity in plants.Science advances · 2022Article
- The LysR-Type Transcriptional Regulator CrgA Negatively Regulates the Flagellar Master RegulatorJournal of bacteriology · 2020Article
- An immune receptor complex evolved in soybean to perceive a polymorphic bacterial flagellin.Nature communications · 2020Article
- Beyond natural: synthetic expansions of botanical form and function.The New phytologist · 2020Review
- Applications of Protein Engineering and Directed Evolution in Plant Research.Plant physiology · 2019Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbe-associated molecular patterns (MAMPs) are molecules, or domains within molecules, that are conserved across microbial taxa and can be recognized by a plant or animal immune system. Although MAMP receptors have evolved to recognize conserved epitopes, the MAMPs in some microbial species or strains have diverged sufficiently to render them unrecognizable by some host immune systems. In this study, we carried out in vitro evolution of the Arabidopsis thaliana flagellin receptor FLAGELLIN-SENSING 2 (FLS2) to isolate derivatives that recognize one or more flagellin peptides from bacteria for which the wild-type Arabidopsis FLS2 confers little or no response. A targeted approach generated amino acid variation at FLS2 residues in a region previously implicated in flagellin recognition. The primary screen tested for elevated response to the canonical flagellin peptide from Pseudomonas aeruginosa, flg22. From this pool, we then identified five alleles of FLS2 that confer modest (quantitatively partial) recognition of an Erwinia amylovora flagellin peptide. Use of this Erwinia-based flagellin peptide to stimulate Arabidopsis plants expressing the resulting FLS2 alleles did not lead to a detectable reduction of virulent P. syringae pv. tomato growth. However, combination of two identified mutations into a single allele further increased FLS2-mediated responses to the E. amylovora flagellin peptide. These studies demonstrate the potential to raise the sensitivity of MAMP receptors toward particular targets.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.