ArticleScientific reports2016
Advanced uracil DNA glycosylase-supplemented real-time reverse transcription loop-mediated isothermal amplification (UDG-rRT-LAMP) method for universal and specific detection of Tembusu virus.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
- An integrated extraction-free LAMP platform with colorimetric, real-time, and immunochromatographic strip detection for rapid male-specific DNA screening in forensic triage.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2026Article
- Development of an RT-ERA-CRISPR/Cas12a assay for duck Tembusu virus.Poultry science · 2026Article
- Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of High-Burden Viral Diseases in Resource-Limited Countries.Pathogens (Basel, Switzerland) · 2026Review
- Development and proof-of-concept evaluation for a low resource compatible Chikungunya virus diagnostic.PLoS neglected tropical diseases · 2025Article
- Development of a Rapid Visual Detection Assay for Duck Tembusu Virus Using RT-LAMP-CRISPR/Cas12a.Animals : an open access journal from MDPI · 2024Article
- A DAMP-Based Assay for Rapid and Affordable Diagnosis of Bacterial Meningitis Agents:International journal of molecular sciences · 2024Article
- Article
- Optimization and comparative analysis of LAMP and PCR techniques for the detection of leptospiral DNA in Golden Syrian hamsters.Veterinary research communications · 2024Article
- Rapid visual detection ofJournal of Zhejiang University. Science. B · 2023Article
- Super-assembly of integrated gold magnetic assay with loop-mediated isothermal amplification for point-of-care testing.Nano research · 2023Article
- Development and pre-clinical evaluation of a Zika virus diagnostic for low resource settings.Frontiers in microbiology · 2023Article
- Colorimetric detection of SARS-CoV-2 by uracil-DNA glycosylase (UDG) reverse transcription loop-mediated isothermal amplification (RT-LAMP).International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2022Article
- A Rapid, Highly Sensitive and Open-Access SARS-CoV-2 Detection Assay for Laboratory and Home Testing.Frontiers in molecular biosciences · 2022Article
- Elimination of Carryover Contamination in Real-Time Reverse Transcriptase Loop-Mediated Isothermal Amplification for Rapid Detection of the SARS-CoV-2 Virus in Point-of-Care Testing.Frontiers in cellular and infection microbiology · 2022Article
- A paper-based colorimetric molecular test for SARS-CoV-2 in saliva.Biosensors & bioelectronics: X · 2021Article
- Article
- Development and validation of a one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of ZIKV in patient samples from Brazil.Scientific reports · 2021Article
- Biotic concerns in generating molecular diagnosis matrixes for 4 avian viruses with emphasis on Marek's disease virus.Journal of virological methods · 2019Review
- Interfacing Pathogen Detection with Smartphones for Point-of-Care Applications.Analytical chemistry · 2019Review
- An improved advanced fragment analysis-based classification and risk stratification of pediatric acute lymphoblastic leukemia.Cancer cell international · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tembusu virus (TMUV) is a mosquito-borne flavivirus which threatens both poultry production and public health. In this study we developed a complete open reading frame alignment-based rRT-LAMP method for the universal detection of TUMV. To prevent false-positive results, the reaction was supplemented with uracil DNA glycosylase (UDG) to eliminate carryover contamination. The detection limit of the newly developed UDG-rRT-LAMP for TMUV was as low as 100 copies/reaction of viral RNA and 1 × 10(0.89) - 1 × 10(1.55) tissue culture infectious dose/100 μL of viruses. There were no cross-reactions with other viruses, and the reproducibility of the assay was confirmed by intra- and inter-assay tests with variability ranging from 0.22-3.33%. The new UDG-rRT-LAMP method for TMUV produced the same results as viral isolation combined with RT-PCR as the "gold standard" in 96.88% of cases for 81 clinical samples from subjects with suspected TMUV infection. The addition of UDG can eliminate as much as 1 × 10(-16) g/reaction of contaminants, which can significantly reduce the likelihood of false-positive results during the rRT-LAMP reaction. Our result indicated that our UDG-rRT-LAMP is a rapid, sensitive, specific, and reliable method that can effectively prevent carryover contamination in the detection of TMUV.
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