ArticlePloS one2016
Flow Cytometric Quantification of Peripheral Blood Cell β-Adrenergic Receptor Density and Urinary Endothelial Cell-Derived Microparticles in Pulmonary Arterial Hypertension.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 20 citations in OpenAlex.
- Extracellular Vesicles: Multimodal Tools for Diagnosis, Prognosis, and Therapy in Respiratory Diseases.Expert reviews in molecular medicine · 2025Review
- Extracellular vesicles in venous thromboembolism and pulmonary hypertension.Journal of nanobiotechnology · 2023Review
- Review
- Building predictive disease models using extracellular vesicle microscale flow cytometry and machine learning.Molecular oncology · 2023Article
- Biomarkers in Pulmonary Arterial Hypertension.Diagnostics (Basel, Switzerland) · 2022Review
- An Overview of Circulating Pulmonary Arterial Hypertension Biomarkers.Frontiers in cardiovascular medicine · 2022Review
- Neurohormonal modulation in pulmonary arterial hypertension.The European respiratory journal · 2021Review
- Characterization and origins of cell-free mitochondria in healthy murine and human blood.Mitochondrion · 2020Article
- Extracellular vesicles: novel communicators in lung diseases.Respiratory research · 2020Review
- Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles.Journal of visualized experiments : JoVE · 2020Article
- Interdependence of hypoxia and β-adrenergic receptor signaling in pulmonary arterial hypertension.American journal of physiology. Lung cellular and molecular physiology · 2019Article
- Article
- New windows into the brain: Central nervous system-derived extracellular vesicles in blood.Progress in neurobiology · 2019Review
- Endothelial microvesicles in hypoxic hypoxia diseases.Journal of cellular and molecular medicine · 2018Review
- Relative quantification of beta-adrenergic receptor in peripheral blood cells using flow cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2018Article
- Extracellular vesicles for liquid biopsy in prostate cancer: where are we and where are we headed?Prostate cancer and prostatic diseases · 2017Review
- Pulmonary arterial hypertension treatment with carvedilol for heart failure: a randomized controlled trial.JCI insight · 2017Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Pulmonary arterial hypertension (PAH) is a heterogeneous disease characterized by severe angiogenic remodeling of the pulmonary artery wall and right ventricular hypertrophy. Thus, there is an increasing need for novel biomarkers to dissect disease heterogeneity, and predict treatment response. Although β-adrenergic receptor (βAR) dysfunction is well documented in left heart disease while endothelial cell-derived microparticles (Ec-MPs) are established biomarkers of angiogenic remodeling, methods for easy large clinical cohort analysis of these biomarkers are currently absent. Here we describe flow cytometric methods for quantification of βAR density on circulating white blood cells (WBC) and Ec-MPs in urine samples that can be used as potential biomarkers of right heart failure in PAH. Biotinylated β-blocker alprenolol was synthesized and validated as a βAR specific probe that was combined with immunophenotyping to quantify βAR density in circulating WBC subsets. Ec-MPs obtained from urine samples were stained for annexin-V and CD144, and analyzed by a micro flow cytometer. Flow cytometric detection of alprenolol showed that βAR density was decreased in most WBC subsets in PAH samples compared to healthy controls. Ec-MPs in urine was increased in PAH compared to controls. Furthermore, there was a direct correlation between Ec-MPs and Tricuspid annular plane systolic excursion (TAPSE) in PAH patients. Therefore, flow cytometric quantification of peripheral blood cell βAR density and urinary Ec-MPs may be useful as potential biomarkers of right ventricular function in PAH.
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