ArticleJournal of structural biology2016
An algorithm for estimation and correction of anisotropic magnification distortion of cryo-EM images without need of pre-calibration.
Article in Journal of structural biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 36 citations in OpenAlex.
- WebCalEM: a browser-based tool for routine and accurate pixel-size calibration in cryo-EM.Acta crystallographica. Section F, Structural biology communications · 2026Article
- WebCalEM: a browser-based tool for routine and accurate pixel size calibration in cryo-EM.bioRxiv : the preprint server for biology · 2026Article
- Structure ofViruses · 2023Article
- prM-reactive antibodies reveal a role for partially mature virions in dengue virus pathogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- High resolution single particle Cryo-EM refinement using JSPR.Progress in biophysics and molecular biology · 2021Review
- Structural Basis of Zika Virus Specific Neutralization in Subsequent Flavivirus Infections.Viruses · 2020Article
- Arrangement of the Polymerase Complexes inside a Nine-Segmented dsRNA Virus.Structure (London, England : 1993) · 2020Article
- Estimation of high-order aberrations and anisotropic magnification from cryo-EM data sets inIUCrJ · 2020Article
- Bypassing pan-enterovirus host factor PLA2G16.Nature communications · 2019Article
- Sub-3 Å apoferritin structure determined with full range of phase shifts using a single position of volta phase plate.Journal of structural biology · 2019Article
- A sequestered fusion peptide in the structure of an HIV-1 transmitted founder envelope trimer.Nature communications · 2019Article
- Structural basis of a potent human monoclonal antibody against Zika virus targeting a quaternary epitope.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Coxsackievirus A10 atomic structure facilitating the discovery of a broad-spectrum inhibitor against human enteroviruses.Cell discovery · 2019Article
- Molecular basis for the acid-initiated uncoating of human enterovirus D68.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Refinement and Analysis of the Mature Zika Virus Cryo-EM Structure at 3.1 Å Resolution.Structure (London, England : 1993) · 2018Article
- Cryo-EM structure of the exocyst complex.Nature structural & molecular biology · 2018Article
- A 3.0-Angstrom Resolution Cryo-Electron Microscopy Structure and Antigenic Sites of Coxsackievirus A6-Like Particles.Journal of virology · 2018Article
- Defocus and magnification dependent variation of TEM image astigmatism.Scientific reports · 2018Article
- Stability of Cucumber Necrosis Virus at the Quasi-6-Fold Axis Affects Zoospore Transmission.Journal of virology · 2017Article
- Atomic cryo-EM structures of viruses.Current opinion in structural biology · 2017Review
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Anisotropic magnification distortion of TEM images (mainly the elliptic distortion) has been recently found as a potential resolution-limiting factor in single particle 3-D reconstruction. Elliptic distortions of ∼1-3% have been reported for multiple microscopes under low magnification settings (e.g., 18,000×), which significantly limited the achievable resolution of single particle 3-D reconstruction, especially for large particles. Here we report a generic algorithm that formulates the distortion correction problem as a generalized 2-D alignment task and estimates the distortion parameters directly from the particle images. Unlike the present pre-calibration methods, our computational method is applicable to all datasets collected at a broad range of magnifications using any microscope without need of additional experimental measurements. Moreover, the per-micrograph and/or per-particle level elliptic distortion estimation in our method could resolve potential distortion variations within a cryo-EM dataset, and further improve the 3-D reconstructions relative to constant-value correction by the pre-calibration methods. With successful applications to multiple datasets and cross-validation with the pre-calibration method, we have demonstrated the validity and robustness of our algorithm in estimating the distortion; correction of the elliptic distortion significantly improved the achievable resolutions by ∼1-3 folds and enabled 3-D reconstructions of multiple viral structures at 2.4-2.6Å resolutions. The resolution limits with elliptic distortion and the amounts of resolution improvements with distortion correction were found to strongly correlate with the product of the particle size and the amount of distortion, which can help assess if elliptic distortion is a major resolution limiting factor for single particle cryo-EM projects.
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