ArticleScientific reports2016
High-throughput screening of filamentous fungi using nanoliter-range droplet-based microfluidics.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed.
- High-throughput droplet screening of filamentous fungi on solid substrates.RSC chemical biology · 2026Article
- A scalable PDMS extraction method for profiling fungal volatile compounds.Fungal biology and biotechnology · 2026Article
- In Vitro Evaluation of Effectiveness of Antifungal Agents Against Macrophomina phaseolina.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Advances in machine learning-enhanced microfluidic cell sorting.Science advances · 2025Review
- Rapid filamentous fungi gene knockout identification through high-throughput droplet microfluidics.Analytica chimica acta · 2025Article
- From specialization to broad adoption: Key trends in droplet microfluidic innovations enhancing accessibility to non-experts.Biomicrofluidics · 2025Article
- Considerations for Domestication of Novel Strains of Filamentous Fungi.ACS synthetic biology · 2025Review
- A Comprehensive Review on Bioactive Molecules and Advanced Microorganism Management Technologies.Current issues in molecular biology · 2024Review
- Directed evolution of material-producing microorganisms.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Droplet Microfluidics for High-Throughput Screening and Directed Evolution of Biomolecules.Micromachines · 2024Review
- Recent Advances in Microfluidics for the Early Detection of Plant Diseases in Vegetables, Fruits, and Grains Caused by Bacteria, Fungi, and Viruses.Journal of agricultural and food chemistry · 2024Review
- A Label-Free Droplet Sorting Platform Integrating Dielectrophoretic Separation for Estimating Bacterial Antimicrobial Resistance.Biosensors · 2024Article
- Deep learning enabled label-free microfluidic droplet classification for single cell functional assays.Frontiers in bioengineering and biotechnology · 2024Article
- Fabricated devices for performing bacterial-fungal interaction experiments across scales.Frontiers in microbiology · 2024Review
- High-throughput droplet microfluidics screening and genome sequencing analysis for improved amylase-producing Aspergillus oryzae.Biotechnology for biofuels and bioproducts · 2023Article
- Combinatory Library of Microorganisms in the Selection of Reductive Activity Applied to a Ketone Mixture: Unexpected Highlighting of an Enantioselective Oxidative Activity.Microorganisms · 2023Article
- Ultrahigh-Throughput Enzyme Engineering and Discovery inChemical reviews · 2023Review
- Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability.Materials today. Bio · 2023Article
- High-throughput screening of microbial strains in large-scale microfluidic droplets.Frontiers in bioengineering and biotechnology · 2023Article
- A microfluidic device for simultaneous detection of enzyme secretion and elongation of a single hypha.Frontiers in microbiology · 2023Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Filamentous fungi are an extremely important source of industrial enzymes because of their capacity to secrete large quantities of proteins. Currently, functional screening of fungi is associated with low throughput and high costs, which severely limits the discovery of novel enzymatic activities and better production strains. Here, we describe a nanoliter-range droplet-based microfluidic system specially adapted for the high-throughput sceening (HTS) of large filamentous fungi libraries for secreted enzyme activities. The platform allowed (i) compartmentalization of single spores in ~10 nl droplets, (ii) germination and mycelium growth and (iii) high-throughput sorting of fungi based on enzymatic activity. A 10(4) clone UV-mutated library of Aspergillus niger was screened based on α-amylase activity in just 90 minutes. Active clones were enriched 196-fold after a single round of microfluidic HTS. The platform is a powerful tool for the development of new production strains with low cost, space and time footprint and should bring enormous benefit for improving the viability of biotechnological processes.
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