ArticleNucleic acids research2016
Multiplexed miRNA northern blots via hybridization chain reaction.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
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- λ-exonuclease-driven split G-quadruplex hybridization-based DNA walking system for sensitive microRNA quantification.Biotechnology letters · 2025Article
- Intramolecular enhanced entropy-driven DNA-Au nanodevice for mRNA imaging in living cells.Mikrochimica acta · 2025Article
- A dual-mode biosensor for microRNA detection based on DNA tetrahedron-gated nanochannels.Mikrochimica acta · 2025Article
- An Autocatalytic Platform Combining a Nonlinear Hybridization Chain Reaction and DNAzyme to Detect microRNA.Bio-protocol · 2024Article
- Integrated triple signal amplification strategy for ultrasensitive electrochemical detection of gastric cancer-related microRNA utilizing MoSJournal of nanobiotechnology · 2024Article
- Ultra-sensitive electrochemiluminescent biosensor for miRNA based on CRISPR/Cas13a trans-cleavage-triggered hybridization chain reaction and magnetic-assisted enrichment.Mikrochimica acta · 2023Article
- An overview of biochemical technologies for the cancer biomarker miR-21 detection.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Review
- Orthometric multicolor encoded hybridization chain reaction amplifiers for multiplexed microRNA profiling in living cells.Chemical science · 2023Article
- Metal-organic frameworks-based biosensor for microRNA detection in prostate cancer cell lines.RSC advances · 2022Article
- Development of a Novel Tissue Blot Hybridization Chain Reaction for the Identification of Plant Viruses.Plants (Basel, Switzerland) · 2022Article
- Signal-On Fluorescence Biosensor for Highly Sensitive Detection of miRNA-21 Based on DNAzyme Assisted Double-Hairpin Molecular Beacon.Biosensors · 2022Article
- A Sensitive Thrombin Aptasensor Based on Target Circulation Strategy.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Article
- Reprogrammable Gel Electrophoresis Detection Assay Using CRISPR-Cas12a and Hybridization Chain Reaction.Analytical chemistry · 2021Article
- Dual-Signal Amplification Strategy for Sensitive MicroRNA Detection Based on Rolling Circle Amplification and Enzymatic Repairing Amplification.ACS omega · 2020Article
- Genetically Encoded Reporter Genes for MicroRNA Imaging in Living Cells and Animals.Molecular therapy. Nucleic acids · 2020Review
- Elucidation of leak-resistance DNA hybridization chain reaction with universality and extensibility.Nucleic acids research · 2020Article
- Bringing MicroRNAs to Light: Methods for MicroRNA Quantification and Visualization in Live Cells.Frontiers in bioengineering and biotechnology · 2020Review
- A nanobiosensor based on graphene oxide and DNA binding dye for multi-microRNAs detection.Bioscience reports · 2019Article
- Research advances in the detection of miRNA.Journal of pharmaceutical analysis · 2019Review
Corrections and comments
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2 authors.
Funding
Abstract
Northern blots enable detection of a target RNA of interest in a biological sample using standard benchtop equipment. miRNAs are the most challenging targets as they must be detected with a single short nucleic acid probe. With existing approaches, it is cumbersome to perform multiplexed blots in which several RNAs are detected simultaneously, impeding the study of interacting regulatory elements. Here, we address this shortcoming by demonstrating multiplexed northern blotting based on the mechanism of hybridization chain reaction (HCR). With this approach, nucleic acid probes complementary to RNA targets trigger chain reactions in which fluorophore-labeled DNA hairpins self-assemble into tethered fluorescent amplification polymers. The programmability of HCR allows multiple amplifiers to operate simultaneously and independently within a blot, enabling straightforward multiplexing. We demonstrate simultaneous detection of three endogenous miRNAs in total RNA extracted from 293T and HeLa cells. For a given target, HCR signal scales linearly with target abundance, enabling relative and absolute quantitation. Using non-radioactive HCR, sensitive and selective miRNA detection is achieved using 2'OMe-RNA probes. The HCR northern blot protocol takes ∼1.5 days independent of the number of target RNAs.
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Registered trials
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