Evidence map›Paper›PMID 27269812›Full record

ArticleJournal of biomolecular screening2016

A Platform to Enable the Pharmacological Profiling of Small Molecules in Gel-Based Electrophoretic Mobility Shift Assays.

Timothy L Foley, Dorjbal Dorjsuren, Thomas S Dexheimer, Michael D Burkart, William C Wight, Anton Simeonov

Abstract read
In one paragraph

Article in Journal of biomolecular screening, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Timothy L Foley1 Division of Preclinical Innovation, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD, USA.
Dorjbal Dorjsuren1 Division of Preclinical Innovation, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD, USA.
Thomas S Dexheimer1 Division of Preclinical Innovation, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD, USA.
Michael D Burkart2 Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, USA.
William C Wight3 Luxon Engineering, LLC, San Diego, CA, USA.
Anton Simeonov1 Division of Preclinical Innovation, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD, USA.

Funding

Small molecule screening programZ01HG200319 · NHGRI · NATIONAL HUMAN GENOME RESEARCH INSTITUTE · PI AUSTIN, CHRISTOPHER P · 2004 to 2008
$32.3M
Discovery of antitubercular PptT inhibitorsR21AI090213 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI BURKART, MICHAEL D. · 2010 to 2011
$407k
A qHTS FRET-Quench Assay for 4?-PhosphopantethienyltransferasesR03MH083266 · NIMH · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI BURKART, MICHAEL D. · 2007 to 2007
$25k
Intramural NIH HHS Z01 HG200319NIAID NIH HHS R21 AI090213NIMH NIH HHS R03 MH083266
6 · The paper itself

Abstract

We describe a polyacrylamide gel casting cassette that overcomes limitations of commercially available gel electrophoresis equipment. This apparatus molds a single polyacrylamide gel that can evaluate more than 200 samples in parallel, is loaded with a multichannel pipettor, and is flexible with respect to composition of the separating matrix. We demonstrate its use to characterize inhibitors of enzymes that modify protein and nucleic acid substrates. Throughputs of greater than 1000 samples per day were achieved when this system was paired with a quantitative laser-based imaging system, yielding data of remarkable quality.

Indexed as

DNA-Binding ProteinsElectrophoretic Mobility Shift AssayEnzyme InhibitorsHigh-Throughput Screening AssaysHumansProtein BindingSmall Molecule LibrariesDNA-Binding ProteinsEnzyme InhibitorsSmall Molecule Librarieselectrophoretic mobility shift assay (EMSA)electrophoretic techniquesenzymatic assays and analyseshigh-throughput screening (HTS)polyacrylamide gel electrophoresis (PAGE)small-molecule testing

Identifiers

PMID27269812
PMCPMC5269537

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.