Evidence map›Paper›PMID 27269204›Full record

ArticleTissue engineering. Part A2016

Hybrid Biomaterial with Conjugated Growth Factors and Mesenchymal Stem Cells for Ectopic Bone Formation.

Xue Yuan, Randall J Smith, Huiyan Guan, Ciprian N Ionita, Parag Khobragade, Rosemary Dziak, Zunpeng Liu, Manhui Pang, Changdong Wang, Guoqiang Guan and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Tissue engineering. Part A, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
4.6field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 28 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Xue Yuan1 Department of Oral Biology, State University of New York , Buffalo, New York.
Randall J Smith2 Department of Biomedical Engineering, State University of New York , Buffalo, New York.
Huiyan Guan1 Department of Oral Biology, State University of New York , Buffalo, New York.
Ciprian N Ionita2 Department of Biomedical Engineering, State University of New York , Buffalo, New York.
Parag Khobragade2 Department of Biomedical Engineering, State University of New York , Buffalo, New York.
Rosemary Dziak1 Department of Oral Biology, State University of New York , Buffalo, New York.
Zunpeng Liu1 Department of Oral Biology, State University of New York , Buffalo, New York.
Manhui Pang5 Clinical and Translational Research Center, State University of New York , Buffalo, New York.
Changdong Wang1 Department of Oral Biology, State University of New York , Buffalo, New York.
Guoqiang Guan3 Department of Orthodontics, State University of New York , Buffalo, New York.
Stelios Andreadis2 Department of Biomedical Engineering, State University of New York , Buffalo, New York.
Shuying Yang1 Department of Oral Biology, State University of New York , Buffalo, New York.
State University of New York · USUniversity at Buffalo, State University of New York · US

Funding

Regulation of skeletal development and homeostasis by IFT proteinR01DE023105 · NIDCR · UNIVERSITY OF PENNSYLVANIA · PI YANG, SHUYING · 2014 to 2018
$2.0M
Role of RGS12, a Regulator of G protein Signaling, in Bone RemodelingR01AR066101 · NIAMS · UNIVERSITY OF PENNSYLVANIA · PI YANG, SHUYING · 2014 to 2018
$1.8M
Function of Regulator of G protein signaling in aging skeletonR01AG048388 · NIA · UNIVERSITY OF PENNSYLVANIA · PI YANG, SHUYING · 2014 to 2018
$1.7M
NIAMS NIH HHS R01 AR066101NIA NIH HHS R01 AG048388NIDCR NIH HHS R01 DE023105
6 · The paper itself

Abstract

Bone is a highly vascularized tissue and efficient bone regeneration requires neovascularization, especially for critical-sized bone defects. We developed a novel hybrid biomaterial comprising nanocalcium sulfate (nCS) and fibrin hydrogel to deliver mesenchymal stem cells (MSCs) and angiogenic factors, vascular endothelial growth factor (VEGF) and fibroblast growth factor 9 (FGF9), to promote neovascularization and bone formation. MSC and growth factor(s)-loaded scaffolds were implanted subcutaneously into mice to examine their angiogenic and osteogenic potential. Micro CT, alkaline phosphatase activity assay, and histological analysis were used to evaluate bone formation, while immunohistochemistry was employed to assess neovessel formation. The presence of fibrin preserved the nCS scaffold structure and promoted de novo bone formation. In addition, the presence of bone morphogenic protein 2-expressing MSC in nCS and fibrin hydrogels improved bone regeneration significantly. While FGF9 alone had no significant effect, the combination FGF9 and VEGF conjugated in fibrin enhanced neovascularization and bone formation more than 4-fold compared to nCS with MSC. Overall, our results suggested that the combination of nCS (to support bone formation) with a fibrin-based VEGF/FGF9 release system (support vascular formation) is an innovative and effective strategy that significantly enhanced ectopic bone formation in vivo.

Indexed as

Calcium SulfateFibroblast Growth Factor 9HydrogelsVascular Endothelial Growth Factor AAnimalsDelayed-Action PreparationsFibrinHumansHuman Umbilical Vein Endothelial CellsImmobilized ProteinsMesenchymal Stem CellsMiceOsteogenesisCalcium SulfateDelayed-Action PreparationsFgf9 protein, mouseFibrinFibroblast Growth Factor 9HydrogelsImmobilized ProteinsVascular Endothelial Growth Factor Avascular endothelial growth factor A, mouse

Identifiers

PMID27269204
PMCPMC4948201
OpenAlexW2415092320

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.