ArticleThe Plant cell2016
CASEIN KINASE1-LIKE PROTEIN2 Regulates Actin Filament Stability and Stomatal Closure via Phosphorylation of Actin Depolymerizing Factor.
Article in The Plant cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed.
- PuCBL9-PuCIPK11 Modulates Stomatal Microfilament Reorganisation in a Phosphorylation-Dependent Manner to Enhance Drought Tolerance in Poplar.Plant, cell & environment · 2026Article
- FaCPK19-FaTT1 axis decodes heat-induced CaThe New phytologist · 2026Article
- Review
- The RCAR12-CAP1-OST1 module controls ABA-mediated stomatal closure in Arabidopsis.PLoS genetics · 2026Article
- FIMBRIN2 Regulates ABA-Induced Stomatal Closure by Promoting Microfilament Bundling and Turnover in Arabidopsis Guard Cells.Plant direct · 2026Article
- Cytoskeletal control of development and environmental adaptation in plants.Science China. Life sciences · 2026Review
- A dual role ofScience advances · 2026Article
- The ADF4-RCAR12 module regulates ABA-mediated drought tolerance inFrontiers in plant science · 2026Article
- Identification ofGenes · 2025Article
- Genome-wide characterization and expression profiling of FIMBRIN gene family members in response to abiotic stress in Medicago sativa.BMC plant biology · 2025Article
- MYB52 negatively regulates ADF9-meditated actin filament bundling in Arabidopsis pavement cell morphogenesis.Journal of integrative plant biology · 2024Article
- Actin-depolymerizing factors 8 and 11 promote root hair elongation at high pH.Plant communications · 2024Article
- ZmADF5, a Maize Actin-Depolymerizing Factor Conferring Enhanced Drought Tolerance in Maize.Plants (Basel, Switzerland) · 2024Article
- Genome-wide identification of actin-depolymerizing factor family genes in melon (Frontiers in plant science · 2024Article
- Melatonin-mediated CcARP1 alters F-actin dynamics by phosphorylation of CcADF9 to balance root growth and salt tolerance in pigeon pea.Plant biotechnology journal · 2024Article
- SCAB1 coordinates sequential CaScience China. Life sciences · 2024Article
- Activation of actin-depolymerizing factor by CDPK16-mediated phosphorylation promotes actin turnover in Arabidopsis pollen tubes.PLoS biology · 2023Article
- An Overview of the Public Health Challenges in Diagnosing and Controlling Human Foodborne Pathogens.Vaccines · 2023Review
- SALT OVERLY SENSITIVE 1 is inhibited by clade D Protein phosphatase 2C D6 and D7 in Arabidopsis thaliana.The Plant cell · 2023Article
- The rubber tree kinome: Genome-wide characterization and insights into coexpression patterns associated with abiotic stress responses.Frontiers in plant science · 2023Article
3 more citing papers are in PubMed but not listed here.
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7 authors.
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Abstract
The opening and closing of stomata are crucial for plant photosynthesis and transpiration. Actin filaments undergo dynamic reorganization during stomatal closure, but the underlying mechanism for this cytoskeletal reorganization remains largely unclear. In this study, we identified and characterized Arabidopsis thaliana casein kinase 1-like protein 2 (CKL2), which responds to abscisic acid (ABA) treatment and participates in ABA- and drought-induced stomatal closure. Although CKL2 does not bind to actin filaments directly and has no effect on actin assembly in vitro, it colocalizes with and stabilizes actin filaments in guard cells. Further investigation revealed that CKL2 physically interacts with and phosphorylates actin depolymerizing factor 4 (ADF4) and inhibits its activity in actin filament disassembly. During ABA-induced stomatal closure, deletion of CKL2 in Arabidopsis alters actin reorganization in stomata and renders stomatal closure less sensitive to ABA, whereas deletion of ADF4 impairs the disassembly of actin filaments and causes stomatal closure to be more sensitive to ABA Deletion of ADF4 in the ckl2 mutant partially recues its ABA-insensitive stomatal closure phenotype. Moreover, Arabidopsis ADFs from subclass I are targets of CKL2 in vitro. Thus, our results suggest that CKL2 regulates actin filament reorganization and stomatal closure mainly through phosphorylation of ADF.
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