Evidence map›Paper›PMID 27268404›Full record

ArticleBMC infectious diseases2016

Evaluation of Xpert® MTB/RIF and Ustar EasyNAT™ TB IAD for diagnosis of tuberculous lymphadenitis of children in Tanzania: a prospective descriptive study.

Maira Bholla, Neema Kapalata, Edward Masika, Hassan Chande, Levan Jugheli, Mohamed Sasamalo, Tracy R Glass, Hans-Peter Beck, Klaus Reither

Open access · goldAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC infectious diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 6 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed, 6 pooled it
3.1field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 6 syntheses or guidelines pooled it, 32 citations in OpenAlex.

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  6. XpertThe Cochrane database of systematic reviews · 2018
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Maira BhollaSwiss Tropical and Public Health Institute, Socinstrasse 57, 4002, Basel, Switzerland. maira.bholla@gmail.com.ORCID 0000-0003-4831-3420
Neema KapalataNational TB and Leprosy Clinic, Temeke District Hospital, P.O. Box 45232, Dar es Salaam, Tanzania.
Edward MasikaNational TB and Leprosy Clinic, Temeke District Hospital, P.O. Box 45232, Dar es Salaam, Tanzania.
Hassan ChandeDepartment of Histopathology, Muhimbili University of Health and Allied Sciences, P.O. Box 65000, Dar es Salaam, Tanzania.
Levan JugheliSwiss Tropical and Public Health Institute, Socinstrasse 57, 4002, Basel, Switzerland.
Mohamed SasamaloIfakara Health Institute, P.O. Box 74, Bagamoyo, Tanzania.
Tracy R GlassSwiss Tropical and Public Health Institute, Socinstrasse 57, 4002, Basel, Switzerland.
Hans-Peter BeckSwiss Tropical and Public Health Institute, Socinstrasse 57, 4002, Basel, Switzerland.
Klaus ReitherSwiss Tropical and Public Health Institute, Socinstrasse 57, 4002, Basel, Switzerland.
Ifakara Health Institute · TZSwiss Tropical and Public Health Institute · CHMuhimbili University of Health and Allied Sciences · TZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundFine needle aspiration biopsy has become a standard approach for diagnosis of peripheral tuberculous lymphadenitis. The aim of this study was to compare the performance of Xpert MTB/RIF and Ustar EasyNAT TB IAD nucleic acid amplification assays, against acid-fast bacilli microscopy, cytology and mycobacterial culture for the diagnosis of TB lymphadenitis in children from a TB-endemic setting in Tanzania.

methodsChildren of 8 weeks to 16 years of age, suspected of having TB lymphadenitis, were recruited at a district hospital in Tanzania. Fine needle aspirates of lymph nodes were analysed using acid-fast bacilli microscopy, liquid TB culture, cytology, Xpert MTB/RIF and EasyNAT. Latent class analysis and comparison against a composite reference standard comprising "culture and/or cytology" was done, to assess the performance of Xpert MTB/RIF and EasyNAT for the diagnosis of TB lymphadenitis.

resultsSeventy-nine children were recruited; 4 were excluded from analysis. Against a composite reference standard of culture and/or cytology, Xpert MTB/RIF and EasyNAT had a sensitivity and specificity of 58 % and 93 %; and 19 % and 100 % respectively. Relative to latent class definitions, cytology had a sensitivity of 100 % and specificity of 94.7 %.

conclusionsCombining clinical assessment, cytology and Xpert MTB/RIF may allow for a rapid and accurate diagnosis of childhood TB lymphadenitis. Larger diagnostic evaluation studies are recommended to validate these findings and on Xpert MTB/RIF to assess its use as a solitary initial test for TB lymphadenitis in children.

Indexed as

AdolescentBiopsy, Fine-NeedleChildChild, PreschoolFemaleHumansInfantLymph NodesMaleMicroscopyMycobacterium tuberculosisNucleic Acid Amplification TechniquesProspective StudiesSensitivity and SpecificityTanzaniaTuberculosis, Lymph NodeChildhoodCultureCytologyFine needle aspirationLymphadenitisMycobacterium tuberculosisNucleic acid amplificationUstar EasyNATXpert MTB/RIFZiehl-Neelsen

Identifiers

PMID27268404
PMCPMC4895953
OpenAlexW2415618464

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.