ArticleBMC research notes2016
Rapid identification of fungi in culture-negative clinical blood and respiratory samples by DNA sequence analyses.
Article in BMC research notes, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 15 citations in OpenAlex.
- Article
- Molecular Characterisation of Fungi Populations in the Chronic Wounds of Filarial Lymphedema Patients in Southern Ghana: A Cross-Sectional Study.Health science reports · 2025Article
- Development of Melting-Curve-Based Real-Time PCR for Differentiating Medically ImportantInternational journal of molecular sciences · 2025Article
- Atypical presentation for a gastrointestinal basidiobolomycosis and rare side effect of Voriconazole: A case report and literature review.Medical mycology case reports · 2025Article
- Rapid and Differential Diagnosis of Sepsis Stages Using an Advanced 3D Plasmonic Bimetallic Alloy Nanoarchitecture-Based SERS Biosensor Combined with Machine Learning for Multiple Analyte Identification.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The Role of the OLM CandID Real-Time PCR in the Invasive Candidiasis Diagnostic Surveillance in Intensive Care Unit Patients.Microorganisms · 2025Article
- Challenges in Management of Uncommon Otologic Fungal Disease - a Case Series and Review of Literature.Indian journal of otolaryngology and head and neck surgery : official publication of the Association of Otolaryngologists of India · 2024Article
- Accurate diagnosis of bronchopulmonaryFrontiers in cellular and infection microbiology · 2023Article
- Characterization and phylogeny of fungi isolated from industrial wastewater using multiple genes.Scientific reports · 2022Article
- Updated Perspectives on the Diagnosis and Management of Onychomycosis.Clinical, cosmetic and investigational dermatology · 2022Review
- Renal ultrasound imaging in a preterm infant with a persistently elevated C reactive protein.BMJ case reports · 2018Article
- Oral Probiotics Alter Healthy Feline Respiratory Microbiota.Frontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundClinical diagnoses of fungal infections often rely upon culture techniques followed by microscopic examination of positive cultures and histopathological specimens. Culturing of microorganisms is prone to false negatives, while microscopy methods can be complicated by atypical phenotypes and organisms that are morphologically indistinguishable in tissues. Delays in diagnoses (or the lack thereof) and inaccurate identification of infectious organisms contribute to increased morbidity and mortality in patients.
methodsTwo-hundred randomized, heterogeneous patient blood and respiratory samples that were culture-negative were tested using polymerase chain reaction (PCR) amplification of internal transcribed spacer regions of ribosomal RNA genes utilizing panfungal primers. Amplicons were sequenced, subjected to sequence similarity searches, and compared using phylogenetic analyses.
resultsThirteen fungal sequences were detected in three whole-blood samples and nine respiratory samples. Bioinformatic analyses were performed which indicated the presence of multiple pathogens and potential pathogens.
conclusionsThe results from this pilot study demonstrate the utility of PCR assays and sequence analyses in clinical tests for fungi to facilitate rapid diagnosis and appropriate treatments to deal with the false negatives from culture results.
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