Evidence map›Paper›PMID 27267713›Full record

ArticleEndocrinology2016

Angiotensin Type-2 Receptors Influence the Activity of Vasopressin Neurons in the Paraventricular Nucleus of the Hypothalamus in Male Mice.

Annette D de Kloet, Soledad Pitra, Lei Wang, Helmut Hiller, David J Pioquinto, Justin A Smith, Colin Sumners, Javier E Stern, Eric G Krause

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
6.4field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 41 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. The ATMolecules (Basel, Switzerland) · 2023
    Review
  6. Review
  7. The Angiotensin ATPharmacological reviews · 2022
    Review
  8. Article
  9. Article
  10. Limbic Neuropeptidergic Modulators of Emotion and Their Therapeutic Potential for Anxiety and Post-Traumatic Stress Disorder.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021
    Review
  11. Review
  12. GABA is a mediator of brain ATHypertension research : official journal of the Japanese Society of Hypertension · 2020
    Review
  13. Review
  14. Brain angiotensin type-1 and type-2 receptors: cellular locations under normal and hypertensive conditions.Hypertension research : official journal of the Japanese Society of Hypertension · 2020
    Article
  15. Review
  16. Angiotensin Type 2 Receptors: Painful, or Not?Frontiers in pharmacology · 2020
    Review
  17. Article
  18. Article
  19. Oxytocin Receptors Are Expressed by Glutamatergic Prefrontal Cortical Neurons That Selectively Modulate Social Recognition.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019
    Article
  20. Dimerization of ATCurrent hypertension reports · 2018
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Annette D de KloetDepartment of Physiology and Functional Genomics (A.D.d.K., D.J.P., C.S.), College of Medicine, University of Florida, Gainesville, Florida 32610; Physiology (S.P., J.E.S.), Medical College of Georgia, Augusta University, Augusta, Georgia 30912; and Department of Pharmacodynamics (L.W., H.H., J.A.S., E.G.K.)., College of Pharmacy, University of Florida, Gainesville, Florida 32610.
Soledad PitraDepartment of Physiology and Functional Genomics (A.D.d.K., D.J.P., C.S.), College of Medicine, University of Florida, Gainesville, Florida 32610; Physiology (S.P., J.E.S.), Medical College of Georgia, Augusta University, Augusta, Georgia 30912; and Department of Pharmacodynamics (L.W., H.H., J.A.S., E.G.K.)., College of Pharmacy, University of Florida, Gainesville, Florida 32610.
Lei WangDepartment of Physiology and Functional Genomics (A.D.d.K., D.J.P., C.S.), College of Medicine, University of Florida, Gainesville, Florida 32610; Physiology (S.P., J.E.S.), Medical College of Georgia, Augusta University, Augusta, Georgia 30912; and Department of Pharmacodynamics (L.W., H.H., J.A.S., E.G.K.)., College of Pharmacy, University of Florida, Gainesville, Florida 32610.
Helmut HillerDepartment of Physiology and Functional Genomics (A.D.d.K., D.J.P., C.S.), College of Medicine, University of Florida, Gainesville, Florida 32610; Physiology (S.P., J.E.S.), Medical College of Georgia, Augusta University, Augusta, Georgia 30912; and Department of Pharmacodynamics (L.W., H.H., J.A.S., E.G.K.)., College of Pharmacy, University of Florida, Gainesville, Florida 32610.
David J PioquintoDepartment of Physiology and Functional Genomics (A.D.d.K., D.J.P., C.S.), College of Medicine, University of Florida, Gainesville, Florida 32610; Physiology (S.P., J.E.S.), Medical College of Georgia, Augusta University, Augusta, Georgia 30912; and Department of Pharmacodynamics (L.W., H.H., J.A.S., E.G.K.)., College of Pharmacy, University of Florida, Gainesville, Florida 32610.
Justin A SmithDepartment of Physiology and Functional Genomics (A.D.d.K., D.J.P., C.S.), College of Medicine, University of Florida, Gainesville, Florida 32610; Physiology (S.P., J.E.S.), Medical College of Georgia, Augusta University, Augusta, Georgia 30912; and Department of Pharmacodynamics (L.W., H.H., J.A.S., E.G.K.)., College of Pharmacy, University of Florida, Gainesville, Florida 32610.
Colin SumnersDepartment of Physiology and Functional Genomics (A.D.d.K., D.J.P., C.S.), College of Medicine, University of Florida, Gainesville, Florida 32610; Physiology (S.P., J.E.S.), Medical College of Georgia, Augusta University, Augusta, Georgia 30912; and Department of Pharmacodynamics (L.W., H.H., J.A.S., E.G.K.)., College of Pharmacy, University of Florida, Gainesville, Florida 32610.
Javier E SternDepartment of Physiology and Functional Genomics (A.D.d.K., D.J.P., C.S.), College of Medicine, University of Florida, Gainesville, Florida 32610; Physiology (S.P., J.E.S.), Medical College of Georgia, Augusta University, Augusta, Georgia 30912; and Department of Pharmacodynamics (L.W., H.H., J.A.S., E.G.K.)., College of Pharmacy, University of Florida, Gainesville, Florida 32610.
Eric G KrauseDepartment of Physiology and Functional Genomics (A.D.d.K., D.J.P., C.S.), College of Medicine, University of Florida, Gainesville, Florida 32610; Physiology (S.P., J.E.S.), Medical College of Georgia, Augusta University, Augusta, Georgia 30912; and Department of Pharmacodynamics (L.W., H.H., J.A.S., E.G.K.)., College of Pharmacy, University of Florida, Gainesville, Florida 32610.
University of Florida · USAugusta University · US

Funding

MIF: A novel CNS regulator of cardiovascular functionR01HL076803 · NHLBI · UNIVERSITY OF FLORIDA · PI SUMNERS, COLIN · 2005 to 2014
$4.0M
Altered CNS Intercellular Signaling Mechanisms in Cardiovascular DiseaseR01HL090948 · NHLBI · UNIVERSITY OF CINCINNATI · PI STERN, JAVIER E · 2008 to 2017
$3.0M
Paraventricular nucleus regulatory mechanisms in stress and hypertensionR01HL093186 · NHLBI · UNIVERSITY OF FLORIDA · PI SCHEUER, DEBORAH A, SUMNERS, COLIN · 2009 to 2013
$2.0M
Central Mechanisms Underlying the Stress Dampening Effects of Acute HypernatremiaR01HL122494 · NHLBI · UNIVERSITY OF FLORIDA · PI KRAUSE, ERIC GERALD · 2015 to 2019
$1.9M
CENTRAL NEURONAL-GLIAL MECHANISMS AND NEUROHUMORAL ACTIVATION IN HYPERTENSIONR01HL112225 · NHLBI · GEORGIA STATE UNIVERSITY · PI STERN, JAVIER E · 2012 to 2015
$1.5M
Novel role of central AT2R in blood pressure regulationR00HL125805 · NHLBI · UNIVERSITY OF FLORIDA · PI DE KLOET, ANNETTE DIANE · 2016 to 2018
$746k
Central Angiotensin receptors and teh neural control of homeostasisR00HL096830 · NHLBI · UNIVERSITY OF FLORIDA · PI KRAUSE, ERIC GERALD · 2011 to 2013
$727k
Central AT1 receptors and the integrated stress response.K99HL096830 · NHLBI · UNIVERSITY OF CINCINNATI · PI KRAUSE, ERIC GERALD · 2009 to 2010
$248k
Novel role of central AT2R in blood pressure regulationK99HL125805 · NHLBI · UNIVERSITY OF FLORIDA · PI DE KLOET, ANNETTE DIANE · 2015 to 2016
$225k
Angiotensin-II, Hypothalamic Inflammation and Neurogenic HypertensionF32HL116074 · NHLBI · UNIVERSITY OF FLORIDA · PI DE KLOET, ANNETTE DIANE · 2012 to 2014
$91k
NHLBI NIH HHS F32 HL116074NHLBI NIH HHS K99 HL096830NHLBI NIH HHS K99 HL125805NHLBI NIH HHS R00 HL096830NHLBI NIH HHS R00 HL125805NHLBI NIH HHS R01 HL076803NHLBI NIH HHS R01 HL090948NHLBI NIH HHS R01 HL093186NHLBI NIH HHS R01 HL112225NHLBI NIH HHS R01 HL122494
6 · The paper itself

Abstract

It is known that angiotensin-II acts at its type-1 receptor to stimulate vasopressin (AVP) secretion, which may contribute to angiotensin-II-induced hypertension. Less well known is the impact of angiotensin type-2 receptor (AT2R) activation on these processes. Studies conducted in a transgenic AT2R enhanced green fluorescent protein reporter mouse revealed that although AT2R are not themselves localized to AVP neurons within the paraventricular nucleus of the hypothalamus (PVN), they are localized to neurons that extend processes into the PVN. In the present set of studies, we set out to characterize the origin, phenotype, and function of nerve terminals within the PVN that arise from AT2R-enhanced green fluorescent protein-positive neurons and synapse onto AVP neurons. Initial experiments combined genetic and neuroanatomical techniques to determine that γ-aminobutyric acid (GABA)ergic neurons derived from the peri-PVN area containing AT2R make appositions onto AVP neurons within the PVN, thereby positioning AT2R to negatively regulate neuroendocrine secretion. Subsequent patch-clamp electrophysiological experiments revealed that selective activation of AT2R in the peri-PVN area using compound 21 facilitates inhibitory (ie, GABAergic) neurotransmission and leads to reduced activity of AVP neurons within the PVN. Final experiments determined the functional impact of AT2R activation by testing the effects of compound 21 on plasma AVP levels. Collectively, these experiments revealed that AT2R expressing neurons make GABAergic synapses onto AVP neurons that inhibit AVP neuronal activity and suppress baseline systemic AVP levels. These findings have direct implications in the targeting of AT2R for disorders of AVP secretion and also for the alleviation of high blood pressure.

Indexed as

AnimalsArginine VasopressinGABAergic NeuronsGreen Fluorescent ProteinsMaleMiceMice, Inbred C57BLMice, TransgenicNeuronsParaventricular Hypothalamic NucleusReceptor, Angiotensin, Type 2SynapsesArginine Vasopressinenhanced green fluorescent proteinGreen Fluorescent ProteinsReceptor, Angiotensin, Type 2

Identifiers

PMID27267713
PMCPMC4967126
OpenAlexW2416929219

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.