ArticleEMBO reports2016
Higher-order assemblies of oligomeric cargo receptor complexes form the membrane scaffold of the Cvt vesicle.
Article in EMBO reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Mechanism of high-mannose N-glycan breakdown and metabolism by Bifidobacterium longum.Nature chemical biology · 2023Article
- In situ structural analysis reveals membrane shape transitions during autophagosome formation.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Structural mechanism of protein recognition by the FW domain of autophagy receptor Nbr1.Nature communications · 2022Article
- The caspase-6-p62 axis modulates p62 droplets based autophagy in a dominant-negative manner.Cell death and differentiation · 2022Article
- Oligomerization of Selective Autophagy Receptors for the Targeting and Degradation of Protein Aggregates.Cells · 2021Review
- Molecular and structural mechanisms of ZZ domain-mediated cargo selection by Nbr1.The EMBO journal · 2021Article
- Autophagy mediates grain yield and nitrogen stress resistance by modulating nitrogen remobilization in rice.PloS one · 2021Article
- Cryo-EM structure of fission yeast tetrameric α-mannosidase Ams1.FEBS open bio · 2020Article
- Recent Advances in Single-Particle Electron Microscopic Analysis of Autophagy Degradation Machinery.International journal of molecular sciences · 2020Review
- p62 filaments capture and present ubiquitinated cargos for autophagy.The EMBO journal · 2018Article
- Distinguishing signal from autofluorescence in cryogenic correlated light and electron microscopy of mammalian cells.Journal of structural biology · 2018Article
- Article
- Review
- Molecular definitions of autophagy and related processes.The EMBO journal · 2017Review
- Next-generation electron microscopy in autophagy research.Current opinion in structural biology · 2016Review
- Higher-order assemblies of oligomeric cargo receptor complexes form the membrane scaffold of the Cvt vesicle.EMBO reports · 2016Article
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Authors and funding
14 authors.
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Abstract
Selective autophagy is the mechanism by which large cargos are specifically sequestered for degradation. The structural details of cargo and receptor assembly giving rise to autophagic vesicles remain to be elucidated. We utilize the yeast cytoplasm-to-vacuole targeting (Cvt) pathway, a prototype of selective autophagy, together with a multi-scale analysis approach to study the molecular structure of Cvt vesicles. We report the oligomeric nature of the major Cvt cargo Ape1 with a combined 2.8 Å X-ray and negative stain EM structure, as well as the secondary cargo Ams1 with a 6.3 Å cryo-EM structure. We show that the major dodecameric cargo prApe1 exhibits a tendency to form higher-order chain structures that are broken upon interaction with the receptor Atg19 in vitro The stoichiometry of these cargo-receptor complexes is key to maintaining the size of the Cvt aggregate in vivo Using correlative light and electron microscopy, we further visualize key stages of Cvt vesicle biogenesis. Our findings suggest that Atg19 interaction limits Ape1 aggregate size while serving as a vehicle for vacuolar delivery of tetrameric Ams1.
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