ArticleCellular microbiology2016
Transcriptomic and molecular genetic analysis of the cell wall salvage response of Aspergillus niger to the absence of galactofuranose synthesis.
Article in Cellular microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.
- Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks.Nucleic acids research · 2019Pooled it
- Rho2 regulates granulocyte-triggered stress adaptation and cell wall remodeling in Aspergillus fumigatus.Microbiological research · 2025Article
- Comprehensive phenotypic analysis of multiple gene deletions of α-glucan synthase and Crh-transglycosylase gene families inCell surface (Amsterdam, Netherlands) · 2025Article
- Cell walls of filamentous fungi - challenges and opportunities for biotechnology.Applied microbiology and biotechnology · 2025Review
- The role of the Flb protein family in the life cycle of Aspergillus niger.Antonie van Leeuwenhoek · 2024Article
- Compatible solutes determine the heat resistance of conidia.Fungal biology and biotechnology · 2023Article
- The α-(1,3)-glucan synthase gene agsE impacts the secretome of Aspergillus niger.Antonie van Leeuwenhoek · 2023Article
- Article
- Conidium Specific Polysaccharides inJournal of fungi (Basel, Switzerland) · 2023Article
- Identification of galactofuranose antigens such as galactomannoproteins and fungal-type galactomannan from the yellowFrontiers in microbiology · 2023Article
- Identification of chitin synthase activator in Aspergillus niger and its application in citric acid fermentation.Applied microbiology and biotechnology · 2022Article
- Effect ofACS omega · 2022Article
- Transcriptome Analysis Reveals Candidate Genes Involved in Light-Induced Primordium Differentiation inInternational journal of molecular sciences · 2021Article
- Article
- Deletion of theMicroorganisms · 2020Article
- Interrogation of the cell wall integrity pathway inGene: X · 2020Article
- A seven-membered cell wall related transglycosylase gene family inCell surface (Amsterdam, Netherlands) · 2020Article
- Functional analysis of three putative galactofuranosyltransferases with redundant functions in galactofuranosylation in Aspergillus niger.Archives of microbiology · 2020Article
- Aspergillus fumigatus establishes infection in zebrafish by germination of phagocytized conidia, while Aspergillus niger relies on extracellular germination.Scientific reports · 2019Article
- FlbA-Regulated GeneApplied and environmental microbiology · 2019Article
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Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The biosynthesis of cell surface-located galactofuranose (Galf)-containing glycostructures such as galactomannan, N-glycans and O-glycans in filamentous fungi is important to secure the integrity of the cell wall. UgmA encodes an UDP-galactopyranose mutase, which is essential for the formation of Galf. Consequently, the ΔugmA mutant lacks Galf-containing molecules. Our previous work in Aspergillus niger work suggested that loss of function of ugmA results in activation of the cell wall integrity (CWI) pathway which is characterized by increased expression of the agsA gene, encoding an α-glucan synthase. In this study, the transcriptional response of the ΔugmA mutant was further linked to the CWI pathway by showing the induced and constitutive phosphorylation of the CWI-MAP kinase in the ΔugmA mutant. To identify genes involved in cell wall remodelling in response to the absence of galactofuranose biosynthesis, a genome-wide expression analysis was performed using RNAseq. Over 400 genes were higher expressed in the ΔugmA mutant compared to the wild-type. These include genes that encode enzymes involved in chitin (gfaB, gnsA, chsA) and α-glucan synthesis (agsA), and in β-glucan remodelling (bgxA, gelF and dfgC), and also include several glycosylphosphatidylinositol (GPI)-anchored cell wall protein-encoding genes. In silico analysis of the 1-kb promoter regions of the up-regulated genes in the ΔugmA mutant indicated overrepresentation of genes with RlmA, MsnA, PacC and SteA-binding sites. The importance of these transcription factors for survival of the ΔugmA mutant was analysed by constructing the respective double mutants. The ΔugmA/ΔrlmA and ΔugmA/ΔmsnA double mutants showed strong synthetic growth defects, indicating the importance of these transcription factors to maintain cell wall integrity in the absence of Galf biosynthesis.
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