ArticleScientific reports2016
Identification and characterization of DcUCGalT1, a galactosyltransferase responsible for anthocyanin galactosylation in purple carrot (Daucus carota L.) taproots.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Diversity and ecological functions of anthocyanins.BMC plant biology · 2025Review
- Article
- Sensory, textural, physico-chemical and enzymatic characterization of melted cheese with added potato and carrot peels.Frontiers in nutrition · 2023Article
- Acylation of Anthocyanins and Their Applications in the Food Industry: Mechanisms and Recent Research Advances.Foods (Basel, Switzerland) · 2022Review
- Glycosyltransferases: Mining, engineering and applications in biosynthesis of glycosylated plant natural products.Synthetic and systems biotechnology · 2022Article
- Origin, evolution, breeding, and omics of Apiaceae: a family of vegetables and medicinal plants.Horticulture research · 2022Article
- Characterization of Two Key Flavonoid 3-Frontiers in plant science · 2022Article
- Contrasting a reference cranberry genome to a crop wild relative provides insights into adaptation, domestication, and breeding.PloS one · 2022Article
- Article
- Anthocyanin synthesis in orange carrot cv. Danvers is activated by transgene expression of the transcription factors DcMYB113_NB and DcEGL1_NB from black carrot cv. Nightbird.Plant molecular biology · 2021Article
- A Roadmap to Modulated Anthocyanin Compositions in Carrots.Plants (Basel, Switzerland) · 2021Review
- Cyanidin 3-International journal of molecular sciences · 2021Review
- Review
- Cyanidin based anthocyanin biosynthesis in orange carrot is restored by expression of AmRosea1 and AmDelila, MYB and bHLH transcription factors.Plant molecular biology · 2020Article
- DcMYB113, a root-specific R2R3-MYB, conditions anthocyanin biosynthesis and modification in carrot.Plant biotechnology journal · 2020Article
- Article
- Isolation, purification and characterization of an ascorbate peroxidase from celery and overexpression of the AgAPX1 gene enhanced ascorbate content and drought tolerance in Arabidopsis.BMC plant biology · 2019Article
- Changing Carrot Color: Insertions inPlant physiology · 2019Article
- Identification of an SCPL Gene Controlling Anthocyanin Acylation in Carrot (Frontiers in plant science · 2019Article
- Advances in research on the carrot, an important root vegetable in the Apiaceae family.Horticulture research · 2019Review
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Purple carrots (Daucus carota ssp. sativus var. atrorubens Alef.) accumulate large amounts of cyanidin-based anthocyanins in their taproots. Cyanidin can be glycosylated with galactose, xylose, and glucose in sequence by glycosyltransferases resulting in cyanidin 3-xylosyl (glucosyl) galactosides in purple carrots. The first step in the glycosylation of cyanidin is catalysis by UDP-galactose: cyanidin galactosyltransferase (UCGalT) transferring the galactosyl moiety from UDP-galactose to cyanidin. In the present study, a gene from 'Deep purple' carrot, DcUCGalT1, was cloned and heterologously expressed in E. coli BL21 (DE3). The recombinant DcUCGalT1 galactosylated cyanidin to produce cyanidin-3-O-galactoside and showed optimal activity for cyanidin at 30 °C and pH 8.6. It showed lower galactosylation activity for peonidin, pelargonidin, kaempferol and quercetin. It accepted only UDP-galactose as a glycosyl donor when cyanidin was used as an aglycone. The expression level of DcUCGalT1 was positively correlated with anthocyanin biosynthesis in carrots. The enzyme extractions from 'Deep purple' exhibited galactosylation activity for cyanidin, peonidin and pelargonidin, while those from 'Kuroda' (a non-purple cultivar) did not.
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