ReviewScandinavian journal of medicine & science in sports2017
An overview of technical considerations for Western blotting applications to physiological research.
Review in Scandinavian journal of medicine & science in sports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 164 papers, 3 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
164 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- Brief guide to western blot assays.Molecules and cells · 2025Guideline
- Factors Influencing AMPK Activation During Cycling Exercise: A Pooled Analysis and Meta-Regression.Sports medicine (Auckland, N.Z.) · 2022Pooled it
- Guidelines for authors and reviewers on antibody use in physiology studies.American journal of physiology. Heart and circulatory physiology · 2018Guideline
- Molecular mechanisms underpinning favourable physiological adaptations to exercise prehabilitation for urological cancer surgery.Prostate cancer and prostatic diseases · 2024Trial
- Shatavari supplementation in postmenopausal women alters the skeletal muscle proteome and pathways involved in training adaptation.European journal of nutrition · 2024Trial
- Pharmacological hypogonadism impairs molecular transducers of exercise-induced muscle growth in humans.Journal of cachexia, sarcopenia and muscle · 2022Trial
- Testosterone therapy induces molecular programming augmenting physiological adaptations to resistance exercise in older men.Journal of cachexia, sarcopenia and muscle · 2019Trial
- Human skeletal muscle is refractory to the anabolic effects of leucine during the postprandial muscle-full period in older men.Clinical science (London, England : 1979) · 2017Trial
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- Cascade-targeted intervention of the NLRP3 inflammasome and downstream angiogenesis by curcumin nanoparticles for ocular neovascularization therapy.Clinical and translational medicine · 2026Article
- A multiscale PBPK-PD model of oxytocin-induced uterine excitation and contraction.The Journal of physiology · 2026Article
- p-Coumaric Acid Attenuates Lead Acetate-induced Neurotoxicity in Rats by Improving Behavioral Dysfunction and Suppressing Oxidative Stress, Neuroinflammation, Apoptosis, and Plasticity-related Molecular Alterations.Biological trace element research · 2026Article
- Comparative sperm proteomics in IVF patients with differential fertilization rates identifies FAM205A as a novel biomarker for male infertility.Asian journal of andrology · 2026Article
- Preparation and Epitope Identification of Monoclonal Antibodies Against African Swine Fever Virus pE120R.Veterinary sciences · 2026Article
- Quantitative western blotting adapted for expanded sample capacity with non-linear curve fitting.Journal of plant research · 2026Article
- mTORC1 signalling and protein synthesis are elevated in response to amino acids in human myotubes obtained from young, old, and old trained men.In vitro cellular & developmental biology. Animal · 2026Article
- A combined strategy of EGFR-MET bispecific antibody and HER3 ADC to overcome osimertinib resistance in NSCLC.Cellular oncology (Dordrecht, Netherlands) · 2026Article
- Modulation of gut microbiota and its metabolite Equol by Huaier granule suppresses hepatocellular carcinoma via the gut-liver axis.NPJ biofilms and microbiomes · 2026Article
- Hesperidin mitigates DEHP-induced nephrotoxicity through anti-oxidant, anti-apoptotic, and anti-inflammatory pathway modulation: Evidence from Nrf2/HO-1/Keap-1, Bax/Bcl-2/caspase-3, and TLR4/NF-κB axis activation.Iranian journal of basic medical sciences · 2026Article
104 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors.
Funding
Abstract
The applications of Western/immunoblotting (WB) techniques have reached multiple layers of the scientific community and are now considered routine procedures in the field of physiology. This is none more so than in relation to skeletal muscle physiology (i.e., resolving the mechanisms underpinning adaptations to exercise). Indeed, the inclusion of WB data is now considered an essential aspect of many such physiological publications to provide mechanistic insight into regulatory processes. Despite this popularity, and due to the ubiquitous and relatively inexpensive availability of WB equipment, the quality of WB in publications and subsequent analysis and interpretation of the data can be variable, perhaps resulting in spurious conclusions. This may be due to poor laboratory technique and/or lack of comprehension of the critical steps involved in WB and what quality control procedures should be in place to ensure robust data generation. The present review aims to provide a detailed description and critique of WB procedures and technicalities, from sample collection through preparation, blotting and detection, to analysis of the data collected. We aim to provide the reader with improved expertise to critically conduct, evaluate, and troubleshoot the WB process, to produce reproducible and reliable blots.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.