Evidence map›Paper›PMID 27263408›Full record

ArticleMethods in cell biology2016

Second harmonic generation microscopy in zebrafish.

D C LeBert, J M Squirrell, A Huttenlocher, K W Eliceiri

Open access · greenAbstract read
In one paragraph

Article in Methods in cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
8.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 24 citations in OpenAlex.

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  12. Toward QuantitativeFrontiers in cell and developmental biology · 2021
    Article
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  15. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

D C LeBertUniversity of Wisconsin-Madison, Madison, WI, United States.
J M SquirrellUniversity of Wisconsin-Madison, Madison, WI, United States.
A HuttenlocherUniversity of Wisconsin-Madison, Madison, WI, United States.
K W EliceiriUniversity of Wisconsin-Madison, Madison, WI, United States; Morgridge Institute for Research, Madison, WI, United States.
University of Wisconsin–Madison · US

Funding

Cell migration and wound repairR35GM118027 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Anna Huttenlocher · 2016 to 2026
$7.5M
Neutrophil Chemotaxis in AutoinflammationR01GM074827 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI HUTTENLOCHER, ANNA · 2005 to 2015
$3.3M
A toolkit for imaging and photo-manipulation of signaling in zebrafishR01GM102924 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI HAHN, KLAUS M., HUTTENLOCHER, ANNA · 2012 to 2015
$2.5M
Graduate Training In Cellular And Molecular Pathogenesis Of Human DiseasesT32GM081061 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI FABRY, ZSUZSANNA · 2010 to 2019
$1.8M
NIGMS NIH HHS R01 GM074827NIGMS NIH HHS R01 GM102924NIGMS NIH HHS R35 GM118027NIGMS NIH HHS T32 GM081061
6 · The paper itself

Abstract

Modern optical imaging has progressed rapidly with the ability to noninvasively image cellular and subcellular phenomena with high spatial and temporal resolution. In particular, emerging techniques such as second harmonic generation (SHG) microscopy can allow for the monitoring of intrinsic contrast, such as that from collagen, in live and fixed samples. When coupled with multiphoton fluorescence microscopy, SHG can be used to image interactions between cells and the surrounding extracellular environment. There is recent interest in using these approaches to study inflammation and wound healing in zebrafish, an important model for studying these processes. In this chapter we present the practical aspects of using second harmonic generation to image interactions between leukocytes and collagen during wound healing in zebrafish.

Indexed as

AnimalsEmbryo, NonmammalianLarvaMicroscopyOptical ImagingWound HealingZebrafishMacrophageMultiphotonSHGWound healingZebrafish

Identifiers

PMID27263408
PMCPMC5489409
OpenAlexW2300243768

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.