Evidence map›Paper›PMID 27263406›Full record

ArticleMethods in cell biology2016

Embryonic cell culture in zebrafish.

C A Ciarlo, L I Zon

Open access · greenAbstract read
In one paragraph

Article in Methods in cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 20 citations in OpenAlex.

  1. Review
  2. Review
  3. Emerging Frontiers in Zebrafish Embryonic and Adult-Derived Cell Lines.International journal of molecular sciences · 2025
    Review
  4. Review
  5. Review
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

C A CiarloHarvard Medical School and Children's Hospital, Boston, MA, United States.
L I ZonChildren's Hospital and Dana Farber Cancer Institute, Boston, MA, United States; Harvard University, Cambridge, MA, United States.
Boston Children's Hospital · USHarvard University Press · US

Funding

Cancer Biology in the ZebrafishR01CA103846 · NCI · CHILDREN'S HOSPITAL BOSTON · PI ZON, LEONARD IRA · 2003 to 2022
$7.5M
EMBRYONIC HEMATOPOIESISR01HL048801 · NHLBI · CHILDREN'S HOSPITAL BOSTON · PI ZON, LEONARD IRA · 1992 to 2016
$5.7M
Howard Hughes Medical InstituteNCI NIH HHS R01 CA103846NHLBI NIH HHS R01 HL048801
6 · The paper itself

Abstract

Zebrafish embryonic cell cultures have many useful properties that make them complementary to intact embryos for a wide range of studies. Embryonic cell cultures allow for maintenance of transient cell populations, control of chemical and mechanical cues received by cells, and facile chemical screening. Zebrafish cells can be cultured in either heterogeneous or homogeneous cultures from a wide range of developmental time points. Here we describe two methods with particular applicability to chemical screening: a method for the culture of blastomeres for directed differentiation toward the myogenic lineage and a method for the culture of neural crest cells in heterogeneous cultures from early somitogenesis embryos.

Indexed as

Cell Culture TechniquesAnimalsBlastomeresEmbryonic DevelopmentEmbryo, NonmammalianNeural CrestZebrafishBlastomereCell cultureChemical screeningEmbryonic stem cellsMyogenic progenitorNeural crest

Identifiers

PMID27263406
PMCPMC6020034
OpenAlexW2480478051

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.