Evidence map›Paper›PMID 27263094›Full record

ArticleGlycoconjugate journal2016

AGE-RAGE interaction in the TGFβ2-mediated epithelial to mesenchymal transition of human lens epithelial cells.

Cibin T Raghavan, Ram H Nagaraj

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Glycoconjugate journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed, 1 pooled it
1.9field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Cibin T RaghavanDepartment of Ophthalmology, University of Colorado School of Medicine, 12800 East 19th Avenue, RC-1 North 5102, Aurora, CO, 80045, USA.
Ram H NagarajDepartment of Ophthalmology, University of Colorado School of Medicine, 12800 East 19th Avenue, RC-1 North 5102, Aurora, CO, 80045, USA. ram.nagaraj@ucdenver.edu.
University of Colorado Denver · US

Funding

Molecular Mechanisms of Protein Crosslinking in the LensR01EY023286 · NEI · UNIVERSITY OF COLORADO DENVER · PI NAGARAJ, RAM H · 2013 to 2022
$2.9M
LENS CAPSULE AND SECONDARY CATARACTR01EY022061 · NEI · UNIVERSITY OF COLORADO DENVER · PI NAGARAJ, RAM H · 2013 to 2017
$1.9M
NEI NIH HHS R01 EY022061NEI NIH HHS R01 EY023286
6 · The paper itself

Abstract

Basement membrane (BM) proteins accumulate chemical modifications with age. One such modification is glycation, which results in the formation of advanced glycation endproducts (AGEs). In a previous study, we reported that AGEs in the human lens capsule (BM) promote the TGFβ2-mediated epithelial-to-mesenchymal transition (EMT) of lens epithelial cells, which we proposed as a mechanism for posterior capsule opacification (PCO) or secondary cataract formation. In this study, we investigated the role of a receptor for AGEs (RAGE) in the TGFβ2-mediated EMT in a human lens epithelial cell line (FHL124). RAGE was present in FHL124 cells, and its levels were unaltered in cells cultured on either native or AGE-modified BM or upon treatment with TGFβ2. RAGE overexpression significantly enhanced the TGFβ2-mediated EMT responses in cells cultured on AGE-modified BM compared with the unmodified matrix. In contrast, treatment of cells with a RAGE antibody or EN-RAGE (an endogenous ligand for RAGE) resulted in a significant reduction in the TGFβ2-mediated EMT response. This was accompanied by a reduction in TGFβ2-mediated Smad signaling and ROS generation. These results imply that the interaction of matrix AGEs with RAGE plays a role in the TGFβ2-mediated EMT of lens epithelial cells and suggest that the blockade of RAGE could be a strategy to prevent PCO and other age-associated fibrosis.

Indexed as

Epithelial-Mesenchymal TransitionCapsule OpacificationCell LineEpithelial CellsExtracellular MatrixGlycation End Products, AdvancedHumansLens, CrystallineReceptor for Advanced Glycation End ProductsTransforming Growth Factor beta2AGER protein, humanGlycation End Products, AdvancedReceptor for Advanced Glycation End ProductsTGFB2 protein, humanTransforming Growth Factor beta2AGEsBasement membraneEN-RAGEEpithelial to mesenchymal transitionPosterior capsule opacificationRAGE

Identifiers

PMID27263094
PMCPMC4975983
OpenAlexW2418552753

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.