ArticleJournal of plant research2016
Identifying sex in non-fertile individuals of the moss Drepanocladus turgescens (Bryophyta: Amblystegiaceae) using a novel molecular approach.
Article in Journal of plant research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed, 9 citations in OpenAlex.
- Life-history characteristics and historical factors are important to explain regional variation in reproductive traits and genetic diversity in perennial mosses.Annals of botany · 2023Article
- Genotypic confirmation of a biased phenotypic sex ratio in a dryland moss using restriction fragment length polymorphisms.Applications in plant sciencesArticle
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sex identification before sexual maturity is notoriously difficult in plants with separate sexes, but is crucial to address many life history related issues. To study the performance of the two sexes in the rarely sexually reproducing dioecious moss Drepanocladus turgescens a molecular sex marker is needed. The female-targeting marker previously developed for D. trifarius and D. lycopodioides amplifies for a few D. turgescens males, which can thus not be distinguished from females. In a significant addition to the earlier developed method we sequenced the portion successfully amplified by the primers PT-3f and PT-3r for six females and three males. Differences between males and females were revealed at five sequence positions. Examination of a total of fourteen females and seven marker amplifying males confirm that females and such males differ consistently at these positions. The usefulness of a previous protocol for moss sex identification is thus extended to another dioecious moss by the addition of a step where a portion of the sex-correlated region is sequenced.
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