ArticleThe FEBS journal2016
RubisCO selection using the vigorously aerobic and metabolically versatile bacterium Ralstonia eutropha.
Article in The FEBS journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- In vivo directed evolution of an ultrafast Rubisco from a semianaerobic environment imparts oxygen resistance.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Synthetic Genetic Elements Enable Rapid Characterization of Inorganic Carbon Uptake Systems inACS synthetic biology · 2025Article
- Unlocking the potential of Cupriavidus necator H16 as a platform for bioproducts production from carbon dioxide.World journal of microbiology & biotechnology · 2024Review
- Stable Platform for Mevalonate Bioproduction from COACS sustainable chemistry & engineering · 2024Article
- Engineering Rubisco to enhance COSynthetic and systems biotechnology · 2024Review
- Article
- Prospects for engineeringEngineering microbiology · 2023Review
- Trends in Research and Development for COACS omega · 2023Review
- Engineering Cupriavidus necator H16 for enhanced lithoautotrophic poly(3-hydroxybutyrate) production from COMicrobial cell factories · 2022Article
- The reliance of glycerol utilization by Cupriavidus necator on COApplied microbiology and biotechnology · 2022Article
- Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for COBiotechnology for biofuels · 2021Review
- Functional reconstitution of a bacterial COeLife · 2020Article
- Phosphoglycolate salvage in a chemolithoautotroph using the Calvin cycle.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Directed Evolution of an Improved Rubisco; In Vitro Analyses to Decipher Fact from Fiction.International journal of molecular sciences · 2019Article
- Selection of Cyanobacterial (mBio · 2019Article
- Applications of Protein Engineering and Directed Evolution in Plant Research.Plant physiology · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
unlabelledRecapturing atmospheric CO2 is key to reducing global warming and increasing biological carbon availability. Ralstonia eutropha is a biotechnologically useful aerobic bacterium that uses the Calvin-Benson-Bassham (CBB) cycle and the enzyme ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) for CO2 utilization, suggesting that it may be a useful host to bioselect RubisCO molecules with improved CO2 -capture capabilities. A host strain of R. eutropha was constructed for this purpose after deleting endogenous genes encoding two related RubisCOs. This strain could be complemented for CO2 -dependent growth by introducing native or heterologous RubisCO genes. Mutagenesis and suppressor selection identified amino acid substitutions in a hydrophobic region that specifically influences RubisCO's interaction with its substrates, particularly O2 , which competes with CO2 at the active site. Unlike most RubisCOs, the R. eutropha enzyme has evolved to retain optimal CO2 -fixation rates in a fast-growing host, despite the presence of high levels of competing O2 . Yet its structure-function properties resemble those of several commonly found RubisCOs, including the higher plant enzymes, allowing strategies to engineer analogous enzymes. Because R. eutropha can be cultured rapidly under harsh environmental conditions (e.g., with toxic industrial flue gas), in the presence of near saturation levels of oxygen, artificial selection and directed evolution studies in this organism could potentially impact efforts toward improving RubisCO-dependent biological CO2 utilization in aerobic environments. ENZYMES: d-ribulose 1,5-bisphosphate carboxylase/oxygenase, EC 4.1.1.39; phosphoribulokinase, EC 2.7.1.19.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.