ArticlePlant physiology2016
The Plasma Membrane H+-ATPase AHA1 Plays a Major Role in Stomatal Opening in Response to Blue Light.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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71 citing papers in PubMed, 146 citations in OpenAlex.
- Evolutionary tuning of the molecular charge state of UBP24 shapes responses to high temperature.Nature plants · 2026Article
- Phosphorylation of WDR48 by phototropins drives starch degradation to promote stomatal opening.Nature communications · 2026Article
- Genome-Wide Identification and Characterization of Stress-Responsive SNARE Genes in Quinoa (Biology · 2026Article
- Humidity-driven ABA depletion determines plant-pathogen competition for leaf water.Nature communications · 2025Article
- The rice BRITTLE CULM 4 gene encodes a membrane protein affecting cellulose synthesis in the secondary cell wall.Plant & cell physiology · 2025Article
- A robust technique for gas-exchange measurements using petiole-cut Arabidopsis leaves in a whole plant chamber.Plant & cell physiology · 2025Article
- The Soil-Plant Continuity of Rare Earth Elements: Insights into an Enigmatic Class of Xenobiotics and Their Interactions with Plant Structures and Processes.Journal of xenobiotics · 2025Review
- Plasma membrane HThe Plant journal : for cell and molecular biology · 2025Article
- Boosting photosynthesis opens new opportunities for agriculture sustainability and circular economy: The BEST-CROP research and innovation action.The Plant journal : for cell and molecular biology · 2025Review
- Integrating QTL mapping and GWAS to decipher the genetic mechanisms behind the calcium contents ofFrontiers in plant science · 2025Article
- TOT3-AHA1 module: its role in fine-tuning stomatal responses.Frontiers in plant science · 2025Review
- Starch metabolism in guard cells: At the intersection of environmental stimuli and stomatal movement.Plant physiology · 2024Review
- Fungal toxin fusicoccin enhances plant growth by upregulating 14-3-3 interaction with plasma membrane HScientific reports · 2024Article
- Article
- Phosphorylation of plasma membrane HNature communications · 2024Article
- Light-induced stomatal opening requires phosphorylation of the C-terminal autoinhibitory domain of plasma membrane HNature communications · 2024Article
- A novel semi-dominant mutation inFrontiers in plant science · 2024Article
- Stomatal responses to VPD utilize guard cell intracellular signaling components.Frontiers in plant science · 2024Article
- Hydrogen sulfide improves salt tolerance through persulfidation of PMA1 in Arabidopsis.Plant cell reports · 2023Article
- Identification and improvement of isothiocyanate-based inhibitors on stomatal opening to act as drought tolerance-conferring agrochemicals.Nature communications · 2023Article
11 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stomata open in response to a beam of weak blue light under strong red light illumination. A blue light signal is perceived by phototropins and transmitted to the plasma membrane H(+)-ATPase that drives stomatal opening. To identify the components in this pathway, we screened for mutants impaired in blue light-dependent stomatal opening. We analyzed one such mutant, provisionally named blus2 (blue light signaling2), and found that stomatal opening in leaves was impaired by 65%, although the magnitude of red light-induced opening was not affected. Blue light-dependent stomatal opening in the epidermis and H(+) pumping in guard cell protoplasts were inhibited by 70% in blus2 Whole-genome resequencing identified a mutation in the AHA1 gene of the mutant at Gly-602. T-DNA insertion mutants of AHA1 exhibited a similar phenotype to blus2; this phenotype was complemented by the AHA1 gene. We renamed blus2 as aha1-10 T-DNA insertion mutants of AHA2 and AHA5 did not show any impairment in stomatal response, although the transcript levels of AHA2 and AHA5 were higher than those of AHA1 in wild-type guard cells. Stomata in ost2, a constitutively active AHA1 mutant, did not respond to blue light. A decreased amount of H(+)-ATPase in aha1-10 accounted for the reduced stomatal blue light responses and the decrease was likely caused by proteolysis of misfolded AHA1. From these results, we conclude that AHA1 plays a major role in blue light-dependent stomatal opening in Arabidopsis and that the mutation made the AHA1 protein unstable in guard cells.
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