ArticleVirulence2017
Heterologous expression of Streptococcus mutans Cnm in Lactococcus lactis promotes intracellular invasion, adhesion to human cardiac tissues and virulence.
Article in Virulence, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Glycosylation of oral bacteria in modulating adhesion and biofilm formation.Journal of oral microbiology · 2025Review
- Microbiota present in combined endodontic-periodontal diseases and its risks for endocarditis.Clinical oral investigations · 2023Article
- Involvement of the Streptococcus mutans PgfE and GalE 4-epimerases in protein glycosylation, carbon metabolism, and cell division.Glycobiology · 2023Article
- Article
- Lactococcus lactis endocarditis and liver abscess in an immunocompetent patient: a case report and review of the literature.Journal of medical case reports · 2023Review
- Amyloid Aggregates Are Localized to the Nonadherent Detached Fraction of Aging Streptococcus mutans Biofilms.Microbiology spectrum · 2022Article
- Review
- Amyloid Aggregation of Streptococcus mutans Cnm Influences Its Collagen-Binding Activity.Applied and environmental microbiology · 2021Article
- Detection of Streptococcus mutans in symptomatic and asymptomatic infected root canals.Clinical oral investigations · 2021Article
- Association of Candida albicans and CbpScientific reports · 2021Article
- Collagen Binding Proteins of Gram-Positive Pathogens.Frontiers in microbiology · 2021Review
- Phenotypic and Genotypic Characterization of Streptococcus mutans Strains Isolated from Endodontic Infections.Journal of endodontics · 2020Article
- The Biology ofMicrobiology spectrum · 2019Article
- Inactivation ofJournal of oral microbiology · 2019Article
- CovR and VicRKX Regulate Transcription of the Collagen Binding Protein Cnm of Streptococcus mutans.Journal of bacteriology · 2018Article
- Whole genome sequence and phenotypic characterization of a CbmMolecular oral microbiology · 2018Article
- Characterization of the pgf operon involved in the posttranslational modification of Streptococcus mutans surface proteins.Scientific reports · 2018Article
- Infective Endocarditis in a Young Adult due toCase reports in medicine · 2018Article
- A New Perspective of an Old Villain: Revisiting Biomarkers of Caries Development.EBioMedicine · 2017Article
- Article
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9 authors at 5 institutions in 2 countries.
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Abstract
In S. mutans, the expression of the surface glycoprotein Cnm mediates binding to extracellular matrix proteins, endothelial cell invasion and virulence in the Galleria mellonella invertebrate model. To further characterize Cnm as a virulence factor, the cnm gene from S. mutans strain OMZ175 was expressed in the non-pathogenic Lactococcus lactis NZ9800 using a nisin-inducible system. Despite the absence of the machinery necessary for Cnm glycosylation, Western blot and immunofluorescence microscopy analyses demonstrated that Cnm was effectively expressed and translocated to the cell wall of L. lactis. Similar to S. mutans, expression of Cnm in L. lactis enabled robust binding to collagen and laminin, invasion of human coronary artery endothelial cells and increased virulence in G. mellonella. Using an ex vivo human heart tissue colonization model, we showed that Cnm-positive strains of either S. mutans or L. lactis outcompete their Cnm-negative counterparts for tissue colonization. Finally, Cnm expression facilitated L. lactis adhesion and colonization in a rabbit model of infective endocarditis. Collectively, our results provide unequivocal evidence that binding to extracellular matrices mediated by Cnm is an important virulence attribute of S. mutans and confirm the usefulness of the L. lactis heterologous system for further characterization of bacterial virulence factors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.