Evidence map›Paper›PMID 27259947›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2016

Mapping Auditory Synaptic Circuits with Photostimulation of Caged Glutamate.

Joshua J Sturm, Tuan Nguyen, Karl Kandler

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.6field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Joshua J SturmDepartment of Otolaryngology, School of Medicine, Ear and Eye Institute, University of Pittsburgh, 3501 Fifth Avenue, Pittsburgh, PA, 15213, USA.
Tuan NguyenDepartment of Physics, The College of New Jersey, Ewing, NJ, 08628, USA.
Karl KandlerDepartment of Otolaryngology, School of Medicine, Ear and Eye Institute, University of Pittsburgh, 3501 Fifth Avenue, Pittsburgh, PA, 15213, USA. kkarl@pitt.edu.
University of Pittsburgh · USCollege of New Jersey · US

Funding

Development of Neuronal Circuits In The Auditory SystemsR01DC004199 · NIDCD · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI KANDLER, KARL · 1999 to 2020
$8.5M
Intrinsic Connectivity of the Auditory Midbrain in a Mouse Model of TinnitusF30DC014177 · NIDCD · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI STURM, JOSHUA JOSEPH · 2014 to 2016
$142k
NIDCD NIH HHS F30 DC014177NIDCD NIH HHS R01 DC004199
6 · The paper itself

Abstract

Photostimulation of neurons with caged glutamate is a viable tool for mapping the strength and spatial distribution of synaptic networks in living brain slices. In photostimulation experiments, synaptic connectivity is assessed by eliciting action potentials in putative presynaptic neurons via focal photolysis of caged glutamate, while measuring postsynaptic responses via intracellular recordings. Two approaches are commonly used for delivering light to small, defined areas in the slice preparation; an optical fiber-based method and a laser-scanning-based method. In this chapter, we outline the technical bases for using photostimulation of caged glutamate to map synaptic circuits, and discuss the advantages and disadvantages of using fiber-based vs. laser-based systems.

Indexed as

Action PotentialsAnimalsBrainBrain MappingGlutamic AcidMiceNeuronsPhotic StimulationPhotolysisRatsSynapsesGlutamic AcidAuditory circuit connectivityFiber-based uncagingGlutamate uncagingLaser scanningPhotostimulationSynaptic input mapping

Identifiers

PMID27259947
PMCPMC5957083
OpenAlexW2411259459

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.