Evidence map›Paper›PMID 27258547›Full record

ArticlePloS one2016

Intratumoral Heterogeneity of MicroRNA Expression in Rectal Cancer.

Anne Haahr Mellergaard Eriksen, Rikke Fredslund Andersen, Boye Schnack Nielsen, Flemming Brandt Sørensen, Ane Lindegaard Appelt, Anders Jakobsen, Torben Frøstrup Hansen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 2 pooled it
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 2 syntheses or guidelines pooled it, 20 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Article
  4. Article
  5. Review
  6. Clinical implications of intratumor heterogeneity: challenges and opportunities.Journal of molecular medicine (Berlin, Germany) · 2020
    Review
  7. Article
  8. Tumour Heterogeneity: The Key Advantages of Single-Cell Analysis.International journal of molecular sciences · 2016
    Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Anne Haahr Mellergaard EriksenDanish Colorectal Cancer Center South, Vejle Hospital, Vejle, Denmark.ORCID http://orcid.org/0000-0003-0134-3374
Rikke Fredslund AndersenDanish Colorectal Cancer Center South, Vejle Hospital, Vejle, Denmark.
Boye Schnack NielsenBioneer A/S, Hørsholm, Denmark.
Flemming Brandt SørensenDanish Colorectal Cancer Center South, Vejle Hospital, Vejle, Denmark.
Ane Lindegaard AppeltDanish Colorectal Cancer Center South, Vejle Hospital, Vejle, Denmark.
Anders JakobsenDanish Colorectal Cancer Center South, Vejle Hospital, Vejle, Denmark.
Torben Frøstrup HansenDanish Colorectal Cancer Center South, Vejle Hospital, Vejle, Denmark.
University of Southern Denmark · DKBioneer (Denmark) · DKUniversity of Copenhagen · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionAn increasing number of studies have investigated microRNAs (miRNAs) as potential markers of diagnosis, treatment and prognosis. So far, agreement between studies has been minimal, which may in part be explained by intratumoral heterogeneity of miRNA expression. The aim of the present study was to assess the heterogeneity of a panel of selected miRNAs in rectal cancer, using two different technical approaches. MATERIALS AND

methodsThe expression of the investigated miRNAs was analysed by real-time quantitative polymerase chain reaction (RT-qPCR) and in situ hybridization (ISH) in tumour specimens from 27 patients with T3-4 rectal cancer. From each tumour, tissue from three different luminal localisations was examined. Inter- and intra-patient variability was assessed by calculating intraclass correlation coefficients (ICCs). Correlations between RT-qPCR and ISH were evaluated using Spearman's correlation.

resultsICCsingle (one sample from each patient) was higher than 50% for miRNA-21 and miRNA-31. For miRNA-125b, miRNA-145, and miRNA-630, ICCsingle was lower than 50%. The ICCmean (mean of three samples from each patient) was higher than 50% for miRNA-21(RT-qPCR and ISH), miRNA-125b (RT-qPCR and ISH), miRNA-145 (ISH), miRNA-630 (RT-qPCR), and miRNA-31 (RT-qPCR). For miRNA-145 (RT-qPCR) and miRNA-630 (ISH), ICCmean was lower than 50%. Spearman correlation coefficients, comparing results obtained by RT-qPCR and ISH, respectively, ranged from 0.084 to 0.325 for the mean value from each patient, and from -0.085 to 0.515 in the section including the deepest part of the tumour.

conclusionIntratumoral heterogeneity may influence the measurement of miRNA expression and consequently the number of samples needed for representative estimates. Our findings with two different methods suggest that one sample is sufficient for adequate assessment of miRNA-21 and miRNA-31, whereas more samples would improve the assessment of miRNA-125b, miRNA-145, and miRNA-630. Interestingly, we found a poor correlation between the expression estimates obtained by RT-qPCR and ISH, respectively.

Indexed as

Gene Expression Regulation, NeoplasticGene Expression ProfilingHumansIn Situ HybridizationIn Vitro TechniquesMicroRNAsRectal NeoplasmsReverse Transcriptase Polymerase Chain ReactionMicroRNAsMIRN145 microRNA, humanMIRN21 microRNA, humanMIRN630 microRNA, human

Identifiers

PMID27258547
PMCPMC4892647
OpenAlexW2419634504

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.