ArticleVaccines2016
Aeromonas hydrophila OmpW PLGA Nanoparticle Oral Vaccine Shows a Dose-Dependent Protective Immunity in Rohu (Labeo rohita).
Article in Vaccines, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 76 citations in OpenAlex.
- Current State of Modern Biotechnological-BasedBioMed research international · 2019Pooled it
- Transcriptional investigation of immune gene expression dynamics in Rohu (Labeo rohita) in response to killed Flavobacterium columnare vaccine.Molecular biology reports · 2025Article
- Immunogenicity and protective efficacy of a native omp34 subunit vaccine againstVeterinary world · 2025Article
- Polylactic-Co-glycolic Acid Polymer-Based Nano-Encapsulation Using Recombinant Maltoporin of Aeromonas hydrophila as Potential Vaccine Candidate.Molecular biotechnology · 2025Article
- Advances and Challenges inVaccines · 2025Review
- Advances in Vaccine Adjuvants for Teleost Fish: Implications for Aquatic Welfare and the Potential of Nanoparticle-Based Formulations.Vaccines · 2024Review
- An Introduction to Relevant Immunology Principles with Respect to Oral Vaccines in Aquaculture.Microorganisms · 2023Review
- Nanovaccines to CombatVaccines · 2023Review
- The Screening of the Protective Antigens ofVaccines · 2023Article
- Current Progress and Challenges in the Study of Adjuvants for Oral Vaccines.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2023Review
- Immunogenicity and protective efficacy of OmpA subunit vaccine againstFrontiers in immunology · 2023Article
- Article
- Histopathology of head kidney tissues in challenged rohu,Fish and shellfish immunology reports · 2021Article
- Protection of Teleost Fish against Infectious Diseases through Oral Administration of Vaccines: Update 2021.International journal of molecular sciences · 2021Review
- Nano and Microparticles as Potential Oral Vaccine Carriers and Adjuvants Against Infectious Diseases.Frontiers in pharmacology · 2021Review
- Application of Outer Membrane Protein-Based Vaccines Against Major Bacterial Fish Pathogens in India.Frontiers in immunology · 2020Review
- Comparative Study on A Novel Pathogen of European Seabass. Diversity ofMicroorganisms · 2019Article
- Teleosts Genomics: Progress and Prospects in Disease Prevention and Control.International journal of molecular sciences · 2018Review
- Specific and Efficient Uptake of Surfactant-Free Poly(Lactic Acid) Nanovaccine Vehicles by Mucosal Dendritic Cells in Adult Zebrafish after Bath Immersion.Frontiers in immunology · 2017Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aeromonas hydrophila is a Gram-negative bacterium that causes high mortality in different fish species and at different growth stages. Although vaccination has significantly contributed to the decline of disease outbreaks in aquaculture, the use of oral vaccines has lagged behind the injectable vaccines due to lack of proven efficacy, that being from primary immunization or by use of boost protocols. In this study, the outer membrane protein W (OmpW) of A. hydrophila was cloned, purified, and encapsulated in poly d,l-lactide-co-glycolic acid (PLGA) nanoparticles (NPs) for oral vaccination of rohu (Labeo rohita Hamilton). The physical properties of PLGA NPs encapsulating the recombinant OmpW (rOmpW) was characterized as having a diameter of 370-375 nm, encapsulation efficiency of 53% and -19.3 mV zeta potential. In vitro release of rOmpW was estimated at 34% within 48 h of incubation in phosphate-buffered saline. To evaluate the efficacy of the NP-rOmpW oral vaccine, two antigen doses were orally administered in rohu with a high antigen (HiAg) dose that had twice the amount of antigens compared to the low antigen (LoAg) dose. Antibody levels obtained after vaccination showed an antigen dose dependency in which fish from the HiAg group had higher antibody levels than those from the LoAg group. The antibody levels corresponded with post challenge survival proportions (PCSPs) and relative percent survival (RPS) in which the HiAg group had a higher PCSP and RPS than the LoAg group. Likewise, the ability to inhibit A. hydrophila growth on trypticase soy agar (TSA) by sera obtained from the HiAg group was higher than that from the LoAg group. Overall, data presented here shows that OmpW orally administered using PLGA NPs is protective against A. hydrophila infection with the level of protective immunity induced by oral vaccination being antigen dose-dependent. Future studies should seek to optimize the antigen dose and duration of oral immunization in rohu in order to induce the highest protection in vaccinated fish.
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Registered trials
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