ArticlePloS one2016
CRISPR-Mediated Triple Knockout of SLAMF1, SLAMF5 and SLAMF6 Supports Positive Signaling Roles in NKT Cell Development.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Influenza virus infection drives upregulation of CD84 across a broad range of immune cells.Clinical & translational immunology · 2026Article
- The hyperinflammatory spectrum: from defects in cytotoxicity to cytokine control.Frontiers in immunology · 2023Review
- Current insights in mouse iNKT and MAIT cell development using single cell transcriptomics data.Seminars in immunology · 2022Review
- Development of αβ T Cells with Innate Functions.Advances in experimental medicine and biology · 2022Article
- SLAM family receptors control pro-survival effectors in germinal center B cells to promote humoral immunity.The Journal of experimental medicine · 2021Article
- T follicular helper cells in germinal center B cell selection and lymphomagenesis.Immunological reviews · 2020Review
- Critical Role for SLAM/SAP Signaling in the Thymic Developmental Programming of IL-17- and IFN-γ-Producing γδ T Cells.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
- SLAM-SAP-Fyn: Old Players with New Roles in iNKT Cell Development and Function.International journal of molecular sciences · 2019Review
- Review
- SLAM receptors foster iNKT cell development by reducing TCR signal strength after positive selection.Nature immunology · 2019Article
- The ins and outs of type I iNKT cell development.Molecular immunology · 2019Review
- The Role of Adaptor Proteins in the Biology of Natural Killer T (NKT) Cells.Frontiers in immunology · 2019Review
- Ly9 (SLAMF3) receptor differentially regulates iNKT cell development and activation in mice.European journal of immunology · 2018Article
- NF-κB Protects NKT Cells from Tumor Necrosis Factor Receptor 1-induced Death.Scientific reports · 2017Article
- Dissection of SAP-dependent and SAP-independent SLAM family signaling in NKT cell development and humoral immunity.The Journal of experimental medicine · 2017Article
- Natural Killer T Cells: An Ecological Evolutionary Developmental Biology Perspective.Frontiers in immunology · 2017Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The SLAM family receptors contribute to diverse aspects of lymphocyte biology and signal via the small adaptor molecule SAP. Mutations affecting SAP lead to X-linked lymphoproliferative syndrome Type 1, a severe immunodysregulation characterized by fulminant mononucleosis, dysgammaglobulinemia, and lymphoproliferation/lymphomas. Patients and mice having mutations affecting SAP also lack germinal centers due to a defect in T:B cell interactions and are devoid of invariant NKT (iNKT) cells. However, which and how SLAM family members contribute to these phenotypes remains uncertain. Three SLAM family members: SLAMF1, SLAMF5 and SLAMF6, are highly expressed on T follicular helper cells and germinal center B cells. SLAMF1 and SLAMF6 are also implicated in iNKT development. Although individual receptor knockout mice have limited iNKT and germinal center phenotypes compared to SAP knockout mice, the generation of multi-receptor knockout mice has been challenging, due to the genomic linkage of the genes encoding SLAM family members. Here, we used Cas9/CRISPR-based mutagenesis to generate mutations simultaneously in Slamf1, Slamf5 and Slamf6. Genetic disruption of all three receptors in triple-knockout mice (TKO) did not grossly affect conventional T or B cell development and led to mild defects in germinal center formation post-immunization. However, the TKO worsened defects in iNKT cells development seen in SLAMF6 single gene-targeted mice, supporting data on positive signaling and potential redundancy between these receptors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.