ArticleHorticulture research2016
A next-generation marker genotyping platform (AmpSeq) in heterozygous crops: a case study for marker-assisted selection in grapevine.
Article in Horticulture research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
36 citing papers in PubMed.
- A rhesus macaque model of α-dystroglycanopathy caused by a POMT1 splice altering variant.Animal models and experimental medicine · 2026Article
- Next-Generation Genotyping: Innovations Driving Plant Genomic Improvement.Life (Basel, Switzerland) · 2026Review
- Mapping and identification of QTLs for low light tolerance traits in grapevine.BMC plant biology · 2025Article
- Article
- Multiple routes to fungicide resistance: Interaction of Cyp51 gene sequences, copy number and expression.Molecular plant pathology · 2024Article
- Phenotyping, genetics, and "-omics" approaches to unravel and introgress enhanced resistance against apple scab (Horticulture research · 2024Article
- Rapid and easy construction of a simplified amplicon sequencing (simplified AmpSeq) library for marker-assisted selection.Scientific reports · 2023Article
- Fully sequencing the cassava full-length cDNA library reveals unannotated transcript structures and alternative splicing events in regions with a high density of single nucleotide variations, insertions-deletions, and heterozygous sequences.Plant molecular biology · 2023Article
- Development and validation of an optimized marker set for genomic selection in southern U.S. rice breeding programs.The plant genome · 2022Article
- Validation of KASP-SNP markers in cassava germplasm for marker-assisted selection of increased carotenoid content and dry matter content.Frontiers in plant science · 2022Article
- Stable QTL for malate levels in ripe fruit and their transferability acrossHorticulture research · 2022Article
- Genetic Diversity of Wild and Cultivated Muscadine Grapes (Frontiers in plant science · 2022Article
- Article
- Multiple independent recombinations led to hermaphroditism in grapevine.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Whole genome resequencing and custom genotyping unveil clonal lineages in 'Malbec' grapevines (Vitis vinifera L.).Scientific reports · 2021Article
- Mining Grapevine Downy Mildew Susceptibility Genes: A Resource for Genomics-Based Breeding and Tailored Gene Editing.Biomolecules · 2021Article
- Integration of advanced technologies for plant variety and cultivar identification.Journal of biosciences · 2021Review
- Discovery of theFrontiers in plant science · 2021Article
- Candidate gene mapping identifies genomic variations in the fire blight susceptibility genes HIPM and DIPM across the Malus germplasm.Scientific reports · 2020Article
- Novel stable QTLs identification for berry quality traits based on high-density genetic linkage map construction in table grape.BMC plant biology · 2020Article
Corrections and comments
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Authors and funding
18 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Marker-assisted selection (MAS) is often employed in crop breeding programs to accelerate and enhance cultivar development, via selection during the juvenile phase and parental selection prior to crossing. Next-generation sequencing and its derivative technologies have been used for genome-wide molecular marker discovery. To bridge the gap between marker development and MAS implementation, this study developed a novel practical strategy with a semi-automated pipeline that incorporates trait-associated single nucleotide polymorphism marker discovery, low-cost genotyping through amplicon sequencing (AmpSeq) and decision making. The results document the development of a MAS package derived from genotyping-by-sequencing using three traits (flower sex, disease resistance and acylated anthocyanins) in grapevine breeding. The vast majority of sequence reads (⩾99%) were from the targeted regions. Across 380 individuals and up to 31 amplicons sequenced in each lane of MiSeq data, most amplicons (83 to 87%) had <10% missing data, and read depth had a median of 220-244×. Several strengths of the AmpSeq platform that make this approach of broad interest in diverse crop species include accuracy, flexibility, speed, high-throughput, low-cost and easily automated analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.