ArticleBiotechnology for biofuels2016
Engineering Bacillus licheniformis for the production of meso-2,3-butanediol.
Article in Biotechnology for biofuels, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 94 citations in OpenAlex.
- EngineeringACS synthetic biology · 2026Article
- Mixed sugars to a multipurpose chemical: perspectives of 2,3-butanediol fermentability in complex lignocellulose hydrolysates.Bioprocess and biosystems engineering · 2026Review
- Engineering of Corynebacterium glutamicum for the enhanced production of optically pure (meso)-2,3-butanediol.Bioresources and bioprocessing · 2026Article
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- Metabolic engineering ofSynthetic and systems biotechnology · 2023Article
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- Regulatory role of cysteines in (2R, 3R)-butanediol dehydrogenase BdhA of Bacillus velezensis strain GH1-13.Journal of microbiology (Seoul, Korea) · 2022Article
- Biological Control Efficacy and Action Mechanism ofFrontiers in microbiology · 2022Article
- Enhanced production of iturin A by strengthening fatty acid synthesis modules inFrontiers in bioengineering and biotechnology · 2022Article
- Metabolic engineering of non-pathogenic microorganisms for 2,3-butanediol production.Applied microbiology and biotechnology · 2021Review
- Marine Polysaccharides: Occurrence, Enzymatic Degradation and Utilization.Chembiochem : a European journal of chemical biology · 2021Review
- C4 Bacterial Volatiles Improve Plant Health.Pathogens (Basel, Switzerland) · 2021Review
- Metabolic Engineering ofFrontiers in bioengineering and biotechnology · 2020Article
- Transcriptional Changes in the Xylose Operon inInternational journal of molecular sciences · 2019Article
- Unraveling the specific regulation of the shikimate pathway for tyrosine accumulation in Bacillus licheniformis.Journal of industrial microbiology & biotechnology · 2019Review
- Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production.Microbial cell factories · 2019Article
- Metabolic engineering ofBiotechnology for biofuels · 2019Article
- In silico exploration of Red Sea Bacillus genomes for natural product biosynthetic gene clusters.BMC genomics · 2018Article
- Genetic Tools and Techniques for Recombinant Expression in Thermophilic Bacillaceae.Microorganisms · 2018Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background2,3-Butanediol (2,3-BD) can be used as a liquid fuel additive to replace petroleum oil, and as an important platform chemical in the pharmaceutical and plastic industries. Microbial production of 2,3-BD by Bacillus licheniformis presents potential advantages due to its GRAS status, but previous attempts to use this microorganism as a chassis strain resulted in the production of a mix of D-2,3-BD and meso-2,3-BD isomers.
resultsThe aim of this work was to develop an engineered strain of B. licheniformis suited to produce the high titers of the pure meso-2,3-BD isomer. Glycerol dehydrogenase (Gdh) was identified as the catalyst for D-2,3-BD biosynthesis from its precursor acetoin in B. licheniformis. The gdh gene was, therefore, deleted from the wild-type strain WX-02 to inhibit the flux of acetoin to D-2,3-BD biosynthesis. The acoR gene involved in acetoin degradation through AoDH ES was also deleted to provide adequate flux from acetoin towards meso-2,3-BD. By re-directing the carbon flux distribution, the double-deletion mutant WX-02ΔgdhΔacoR produced 28.2 g/L of meso-2,3-BD isomer with >99 % purity. The titer was 50 % higher than that of the wide type. A bench-scale fermentation by the double-deletion mutant was developed to further improve meso-2,3-BD production. In a fed-batch fermentation, meso-2,3-BD titer reached 98.0 g/L with a purity of >99.0 % and a productivity of 0.94 g/L-h.
conclusionsThis work demonstrates the potential of producing meso-2,3-BD with high titer and purity through metabolic engineering of B. licheniformis.
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