ArticleNucleic acids research2016
Synergy and antagonism in regulation of recombinant human INO80 chromatin remodeling complex.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 39 citations in OpenAlex.
- Recognition and remodelling of nucleosomes and hexasomes by the human INO80 complex.Nucleic acids research · 2026Article
- The Ies6 subunit is essential for INO80-mediated nucleosome organization.Scientific reports · 2026Article
- MacroH2A-Mediated Gene Repression through Nucleosome Compaction and Remodeling Inhibition.bioRxiv : the preprint server for biology · 2026Article
- Integrated Protocol for γTuRC Expression and Purification for Microtubule Nucleation and Cryo-EM Analysis.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Chromatin remodeling complexes: architects influencing breast cancer progression.Frontiers in cell and developmental biology · 2025Review
- Molecular basis of global promoter sensing and nucleosome capture by the SWR1 chromatin remodeler.Cell · 2024Article
- Structural and Biochemical Characterization of the Nucleosome Containing Variants H3.3 and H2A.Z.Epigenomes · 2024Article
- Structural insights into histone exchange by human SRCAP complex.Cell discovery · 2024Article
- New insights into the mechanism and DNA-sequence specificity of INO80 chromatin remodeling.Research square · 2023Article
- Sophisticated Conversations between Chromatin and Chromatin Remodelers, and Dissonances in Cancer.International journal of molecular sciences · 2021Review
- Mechanistic Insights into Regulation of the ALC1 Remodeler by the Nucleosome Acidic Patch.Cell reports · 2020Article
- INO80 and SWR1 complexes: the non-identical twins of chromatin remodelling.Current opinion in structural biology · 2020Review
- Structural analyses of inositol phosphate second messengers bound to signaling effector proteins.Advances in biological regulation · 2020Review
- The Emerging Roles of ATP-Dependent Chromatin Remodeling Complexes in Pancreatic Cancer.Cancers · 2019Review
- Article
- Structure and dynamics of the yeast SWR1-nucleosome complex.Science (New York, N.Y.) · 2018Article
- The Arp8 and Arp4 module acts as a DNA sensor controlling INO80 chromatin remodeling.Nature communications · 2018Article
- Article
- Article
- Identification of Two Distinct Classes of the Human INO80 Complex Genome-Wide.G3 (Bethesda, Md.) · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We have purified a minimal core human Ino80 complex from recombinant protein expressed in insect cells. The complex comprises one subunit each of an N-terminally truncated Ino80, actin, Arp4, Arp5, Arp8, Ies2 and Ies6, together with a single heterohexamer of the Tip49a and Tip49b proteins. This core complex has nucleosome sliding activity that is similar to that of endogenous human and yeast Ino80 complexes and is also inhibited by inositol hexaphosphate (IP6). We show that IP6 is a non-competitive inhibitor that acts by blocking the stimulatory effect of nucleosomes on the ATPase activity. The IP6 binding site is located within the C-terminal region of the Ino80 subunit. We have also prepared complexes lacking combinations of Ies2 and Arp5/Ies6 subunits that reveal regulation imposed by each of them individually and synergistically that couples ATP hydrolysis to nucleosome sliding. This coupling between Ies2 and Arp5/Ies6 can be overcome in a bypass mutation of the Arp5 subunit that is active in the absence of Ies2. These studies reveal several underlying mechanisms for regulation of ATPase activity involving a complex interplay between these protein subunits and IP6 that in turn controls nucleosome sliding.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.