ArticleScientific reports2016
Discovery of a rhamnose utilization pathway and rhamnose-inducible promoters in Pichia pastoris.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Yarrowia lipolytica and Komagataella phaffii (Pichia pastoris) as Hosts for Heterologous Expression of Lipases.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Development and Application of a Cumate-Inducible Promoter, PMicrobial biotechnology · 2026Article
- Transcriptional regulation: efficient genetic engineering tools for non-conventional yeasts.FEMS yeast research · 2026Review
- Next-generation stress-inducible Komagataella phaffii promoter variants.Microbial cell factories · 2025Article
- Rational design and characterization of enhanced alcohol-inducible synthetic promoters inApplied and environmental microbiology · 2025Article
- Enhanced Expression of Alcohol Dehydrogenase I inMicroorganisms · 2023Article
- Genetic tools for metabolic engineering ofEngineering microbiology · 2023Review
- Systematic sequence engineering enhances the induction strength of the glucose-regulated GTH1 promoter of Komagataella phaffii.Nucleic acids research · 2023Article
- Advances in Metabolic Engineering ofJournal of fungi (Basel, Switzerland) · 2023Review
- Second generation Pichia pastoris strain and bioprocess designs.Biotechnology for biofuels and bioproducts · 2022Review
- High-efficiency secretory expression and characterization of the recombinant type III human-like collagen in Pichia pastoris.Bioresources and bioprocessing · 2022Article
- Characterizing and engineering promoters for metabolic engineering ofSynthetic and systems biotechnology · 2022Article
- A programmable high-expression yeast platform responsive to user-defined signals.Science advances · 2022Article
- What makes Komagataella phaffii non-conventional?FEMS yeast research · 2021Review
- Catabolism of L-rhamnose in A. nidulans proceeds via the non-phosphorylated pathway and is glucose repressed by a CreA-independent mechanism.Microbial cell factories · 2020Article
- Article
- Basal transcription profiles of the rhamnose-inducible promoter PCurrent genetics · 2019Article
- Decreased Rhamnose Metabolic Flux Improved Production of Target Proteins and Cell Flocculation inFrontiers in microbiology · 2018Article
- A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastoris.Microbial cell factories · 2017Article
- Molecular strategies to increase the levels of heterologous transcripts in Komagataella phaffii for protein production.Bioengineered · 2017Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rhamnose utilization pathway in Pichia pastoris has not been clarified although this strain can grow well on rhamnose as a sole carbon source. In this study, four genes, PAS_chr4_0338, PAS_chr4_0339, PAS_chr4_0340, and PAS_chr4_0341, were, for the first time, predicted to be involved in rhamnose metabolism along with the previously identified gene PAS_chr1_4-0075. Moreover, expression of these genes, especially PAS_chr4_0341 and PAS_chr1_4-0075 designated as LRA4 and LRA3, was confirmed to significantly increase and clearly decrease in the presences of rhamnose and glucose, respectively. LRA4 encoding a putative L-2-keto-3-deoxyrhamnonate aldolase, was further confirmed via gene disruption and gene complementation to participate in rhamnose metabolism. Using β-galactosidase and green fluorescent protein as reporters, the promoters of LRA4 and LRA3 performed well in driving efficient production of heterologous proteins. By using food grade rhamnose instead of the toxic compound methanol as the inducer, the two promoters would be excellent candidates for driving the production of food-grade and therapeutically important recombinant proteins.
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