ArticleJournal of Zhejiang University. Science. B2016
Magnolol pretreatment attenuates heat stress-induced IEC-6 cell injury.
Article in Journal of Zhejiang University. Science. B, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 13 citations in OpenAlex.
- The Involvement of the PI3K/AKT Pathway in Zn Alleviation of Heat Stress-Induced Damage to Broiler Jejunal Organoids.Animals : an open access journal from MDPI · 2026Article
- Zinc proteinate alleviates thermal stress-induced damage to broiler jejunal organoid integrity and barrier function possibly by promoting cell proliferation via GPR39/PLCβ1-mediated PI3K/AKT and MAPK signaling pathways.Journal of animal science · 2025Article
- Magnolol additive improves growth performance of Linwu ducklings by modulating antioxidative status.PloS one · 2021Article
- Isolation, culture, and identification of duck intestinal epithelial cells and oxidative stress model constructed.In vitro cellular & developmental biology. Animal · 2019Article
- Insights on the Multifunctional Activities of Magnolol.BioMed research international · 2019Review
- Heat stress induces intestinal injury through lysosome- and mitochondria-dependent pathway in vivo and in vitro.Oncotarget · 2017Article
- Magnolol additive as a replacer of antibiotic enhances the growth performance ofAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2017Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveHeat stress (HS) is an important environmental stressor that adversely influences livestock during the summer. The aim of this study was to investigate whether magnolol protects against HS-induced intestinal epithelial cell injury. MATERIALS AND
methodsAn intestinal epithelial cell line (IEC-6) was subjected to HS at 42 °C, with and without magnolol pretreatment. Cell injury was detected by monitoring lactate dehydrogenase (LDH) release. MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay was used to assess cell proliferation and viability, including identifying effective concentrations of magnolol. Flow cytometry confirmed G1-phase cell-cycle arrest and its alleviation by magnolol. Active DNA synthesis was measured by incorporation of nucleic acid 5-ethynyl-2'-deoxyuridine (EdU). G1-phase cell-cycle-related gene expression was assessed by real-time reverse transcription polymerase chain reaction (RT-PCR) and levels of G1-phase-related proteins by Western blotting.
resultsHS induced IEC-6 cell injury and decreased cell viability, as demonstrated by data from LDH and MTS assays, respectively. Based on a number of criteria, IEC-6 cells subjected to HS were arrested in the G1 phase of the cell cycle. Magnolol pretreatment decreased HS-induced cell injury through relief of this cell-cycle arrest.
conclusionsMagnolol pretreatment attenuates HS-induced injury in IEC-6 cells. Magnolol is potentially promising as a protective strategy for HS in livestock.
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