ArticleAdvances in experimental medicine and biology2016
Tethered Function Assays as Tools to Elucidate the Molecular Roles of RNA-Binding Proteins.
Article in Advances in experimental medicine and biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 47 citations in OpenAlex.
- ADAR1 and ADAR2 associate with the RNA exosome and modulate RNA stability.Nucleic acids research · 2026Article
- Engineering a human-based translational activator for targeted protein expression restoration.Nucleic acids research · 2026Article
- Orthogonal RNA-regulated destabilization domains for three-color RNA imaging with minimal RNA perturbation.Nature methods · 2026Article
- Functional Proximity across an mRNA.Biochemistry · 2025Article
- Human Synthetic Biology and Programmable Gene Regulation Control.Annual review of genomics and human genetics · 2025Review
- An inducible tethered function assay reveals differential regulatory effects for the RNA-binding protein YBX3 and its domains.bioRxiv : the preprint server for biology · 2025Article
- Large-scale evaluation of the ability of RNA-binding proteins to activate exon inclusion.Nature biotechnology · 2024Article
- RNA-binding proteins in cardiovascular biology and disease: the beat goes on.Nature reviews. Cardiology · 2024Review
- SUMOylation-Driven mRNA Circularization Enhances Translation and Promotes Lymphatic Metastasis of Bladder Cancer.Cancer research · 2024Article
- Surveying the global landscape of post-transcriptional regulators.Nature structural & molecular biology · 2023Article
- Building Blocks of Artificial CRISPR-Based Systems beyond Nucleases.International journal of molecular sciences · 2022Review
- A plant tethering system for the functional study of protein-RNA interactions in vivo.Plant methods · 2022Article
- Tethered Function Assays to Elucidate the Role of RNA-Binding Proteins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The parent-of-origin lncRNA MISSEN regulates rice endosperm development.Nature communications · 2021Article
- Tagging and Capturing of Lentiviral Vectors Using Short RNAs.International journal of molecular sciences · 2021Article
- C. elegans germ granules require both assembly and localized regulators for mRNA repression.Nature communications · 2021Article
- Large-scale tethered function assays identify factors that regulate mRNA stability and translation.Nature structural & molecular biology · 2020Article
- CRISPR artificial splicing factors.Nature communications · 2020Article
- A non-canonical role for the EDC4 decapping factor in regulating MARF1-mediated mRNA decay.eLife · 2020Article
- Various strategies of effector accumulation to improve the efficiency of genome editing and derivative methodologies.In vitro cellular & developmental biology. Animal · 2020Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Dynamic regulation of RNA molecules is critical to the survival and development of cells. Messenger RNAs are transcribed in the nucleus as intron-containing pre-mRNAs and bound by RNA-binding proteins, which control their fate by regulating RNA stability, splicing, polyadenylation, translation, and cellular localization. Most RBPs have distinct mRNA-binding and functional domains; thus, the function of an RBP can be studied independently of RNA-binding by artificially recruiting the RBP to a reporter RNA and then measuring the effect of RBP recruitment on reporter splicing, stability, translational efficiency, or intracellular trafficking. These tethered function assays therefore do not require prior knowledge of the RBP's endogenous RNA targets or its binding sites within these RNAs. Here, we provide an overview of the experimental strategy and the strengths and limitations of common tethering systems. We illustrate specific examples of the application of the assay in elucidating the function of various classes of RBPs. We also discuss how classic tethering assay approaches and insights gained from them have been empowered by more recent technological advances, including efficient genome editing and high-throughput RNA-sequencing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.