ArticleScientific reports2016
Drosophila cells use nanotube-like structures to transfer dsRNA and RNAi machinery between cells.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Centrosome‑, mitotic spindle‑ and cytokinetic bridge‑specific compartmentalization of AGO2 protein in human liver cells undergoing mitosis: Non‑canonical, RNAi‑dependent, control of local homeostasis.Molecular medicine reports · 2025Article
- Recent advances in understanding of the mechanisms of RNA interference in insects.Insect molecular biology · 2025Review
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- Cell-Autonomous and Non-Cell-Autonomous Antiviral Immunity via siRNA-Directed RNAi inImmune discovery · 2025Article
- RNA interference protocols for gene silencing in the spittlebug Philaenus spumarius, vector of Xylella fastidiosa.Scientific reports · 2024Article
- Review
- Nanotechnology-enabled gene delivery for cancer and other genetic diseases.Expert opinion on drug delivery · 2023Review
- An intra-cytoplasmic route for SARS-CoV-2 transmission unveiled by Helium-ion microscopy.Scientific reports · 2022Article
- Plant and animal small RNA communications between cells and organisms.Nature reviews. Molecular cell biology · 2022Review
- AGO2 localizes to cytokinetic protrusions in a p38-dependent manner and is needed for accurate cell division.Communications biology · 2021Article
- RNAs on the Go: Extracellular Transfer in Insects with Promising Prospects for Pest Management.Plants (Basel, Switzerland) · 2021Review
- Sending Out Alarms: A Perspective on Intercellular Communications in Insect Antiviral Immune Response.Frontiers in immunology · 2021Review
- Conservation of Cell Communication Systems in Invertebrate Host-Defence Mechanisms: Possible Role in Immunity and Disease.Biology · 2020Review
- Mechanisms, Applications, and Challenges of Insect RNA Interference.Annual review of entomology · 2020Review
- Double-Stranded RNA Technology to Control Insect Pests: Current Status and Challenges.Frontiers in plant science · 2020Review
- Management of Pest Insects and Plant Diseases by Non-Transformative RNAi.Frontiers in plant science · 2019Review
- Defense Mechanisms against Viral Infection inViruses · 2018Review
- RNA Interference in Insects: Protecting Beneficials and Controlling Pests.Frontiers in physiology · 2018Review
- The Fly Way of Antiviral Resistance and Disease Tolerance.Advances in immunology · 2018Review
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tunnelling nanotubes and cytonemes function as highways for the transport of organelles, cytosolic and membrane-bound molecules, and pathogens between cells. During viral infection in the model organism Drosophila melanogaster, a systemic RNAi antiviral response is established presumably through the transport of a silencing signal from one cell to another via an unknown mechanism. Because of their role in cell-cell communication, we investigated whether nanotube-like structures could be a mediator of the silencing signal. Here, we describe for the first time in the context of a viral infection the presence of nanotube-like structures in different Drosophila cell types. These tubules, made of actin and tubulin, were associated with components of the RNAi machinery, including Argonaute 2, double-stranded RNA, and CG4572. Moreover, they were more abundant during viral, but not bacterial, infection. Super resolution structured illumination microscopy showed that Argonaute 2 and tubulin reside inside the tubules. We propose that nanotube-like structures are one of the mechanisms by which Argonaute 2, as part of the antiviral RNAi machinery, is transported between infected and non-infected cells to trigger systemic antiviral immunity in Drosophila.
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