ArticleScientific reports2016
Using metabarcoding to reveal and quantify plant-pollinator interactions.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
48 citing papers in PubMed, 1 synthesis or guideline pooled it.
- How quantitative is metabarcoding: A meta-analytical approach.Molecular ecology · 2019Pooled it
- The magnet species effect of two-leaf squill (Frontiers in plant science · 2026Article
- Effect of formic acid treatment on Apis mellifera foraging behavior using nanopore metabarcoding technologies.PloS one · 2026Article
- The Appalbees menu: a multiyear, multilocus metagenetic assessment of pollen foraging by AppalachianPeerJ · 2026Article
- Pollen Foraging by Bumble Bee Queens During a Critical Nesting Period Revealed by DNA Metabarcoding.Ecology and evolution · 2025Article
- Comparing the spring diet of buff-tailed bumble bees and honey bees (Hymenoptera: Apidae) in a forest landscape: a metabarcoding approach.Journal of insect science (Online) · 2025Article
- Authentication of the Botanical Origin of Honey:Journal of agricultural and food chemistry · 2025Article
- Comparative Analysis of Pasture Composition: DNA Metabarcoding Versus Quadrat-Based Botanical Surveys in Experimental Grassland Plots.Ecology and evolution · 2025Article
- A Novel Approach for Pollen Identification and Quantification Using Hybrid Capture-Based DNA Metabarcoding.Ecology and evolution · 2025Article
- Pot-pollen DNA barcoding as a tool to determine the diversity of plant species visited by Ecuadorian stingless bees.PloS one · 2025Article
- Identification of potential insect ecological interactions using a metabarcoding approach.PeerJ · 2025Article
- Uncovering floral composition of paper wasp nests (Hymenoptera: Vespidae: Polistes) through DNA metabarcoding.Scientific reports · 2024Article
- Environmental metagenetics unveil novel plant-pollinator interactions.Ecology and evolution · 2023Article
- Negative effects of urbanisation on diurnal and nocturnal pollen-transport networks.Ecology letters · 2023Article
- Investigating the use of pollen DNA metabarcoding to quantify bee foraging and effects of threshold selection.PloS one · 2023Article
- DNA metabarcoding identifies urban foraging patterns of oligolectic and polylectic cavity-nesting bees.Oecologia · 2022Article
- Honey DNA metabarcoding revealed foraging resource partitioning between Korean native and introduced honey bees (Hymenoptera: Apidae).Scientific reports · 2022Article
- Biomonitoring via DNA metabarcoding and light microscopy of bee pollen in rainforest transformation landscapes of Sumatra.BMC ecology and evolution · 2022Article
- Evaluating restoration trajectories using DNA metabarcoding of ground-dwelling and airborne invertebrates and associated plant communities.Molecular ecology · 2022Article
- Strategies for molecular authentication of herbal products: from experimental design to data analysis.Chinese medicine · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Given the ongoing decline of both pollinators and plants, it is crucial to implement effective methods to describe complex pollination networks across time and space in a comprehensive and high-throughput way. Here we tested if metabarcoding may circumvent the limits of conventional methodologies in detecting and quantifying plant-pollinator interactions. Metabarcoding experiments on pollen DNA mixtures described a positive relationship between the amounts of DNA from focal species and the number of trnL and ITS1 sequences yielded. The study of pollen loads of insects captured in plant communities revealed that as compared to the observation of visits, metabarcoding revealed 2.5 times more plant species involved in plant-pollinator interactions. We further observed a tight positive relationship between the pollen-carrying capacities of insect taxa and the number of trnL and ITS1 sequences. The number of visits received per plant species also positively correlated to the number of their ITS1 and trnL sequences in insect pollen loads. By revealing interactions hard to observe otherwise, metabarcoding significantly enlarges the spatiotemporal observation window of pollination interactions. By providing new qualitative and quantitative information, metabarcoding holds great promise for investigating diverse facets of interactions and will provide a new perception of pollination networks as a whole.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.