ArticleScientific reports2016
Extensive coronavirus-induced membrane rearrangements are not a determinant of pathogenicity.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Perturbation ofJournal of virology · 2025Article
- Identification of Host Proteins Interacting with IBV S1 Based on Tracheal Organ Culture.Viruses · 2023Article
- A little less aggregation a little more replication: Viral manipulation of stress granules.Wiley interdisciplinary reviews. RNA · 2023Review
- Near-Native Visualization of SARS-CoV-2 Induced Membrane Remodeling and Virion Morphogenesis.Viruses · 2022Article
- Flavivirus-host interactions: an expanding network of proviral and antiviral factors.Current opinion in virology · 2022Review
- Article
- Discovery of re-purposed drugs that slow SARS-CoV-2 replication in human cells.PLoS pathogens · 2021Article
- Ultrastructural modifications induced by SARS-CoV-2 in Vero cells: a kinetic analysis of viral factory formation, viral particle morphogenesis and virion release.Cellular and molecular life sciences : CMLS · 2021Article
- Evidences for lipid involvement in SARS-CoV-2 cytopathogenesis.Cell death & disease · 2021Article
- Coronavirus biology and replication: implications for SARS-CoV-2.Nature reviews. Microbiology · 2021Review
- The SARS-Coronavirus Infection Cycle: A Survey of Viral Membrane Proteins, Their Functional Interactions and Pathogenesis.International journal of molecular sciences · 2021Review
- Review
- SARS-CoV-2 Disrupts Splicing, Translation, and Protein Trafficking to Suppress Host Defenses.Cell · 2020Article
- Subdomain cryo-EM structure of nodaviral replication protein A crown complex provides mechanistic insights into RNA genome replication.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A unifying structural and functional model of the coronavirus replication organelle: Tracking down RNA synthesis.PLoS biology · 2020Article
- Extracellular Vesicles in Viral Infections: Two Sides of the Same Coin?Frontiers in cellular and infection microbiology · 2020Review
- The Preparation of Chicken Kidney Cell Cultures for Virus Propagation.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Article
- Membrane binding proteins of coronaviruses.Future virology · 2019Review
- Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Positive-strand RNA (+RNA) viruses rearrange cellular membranes during replication, possibly in order to concentrate and arrange viral replication machinery for efficient viral RNA synthesis. Our previous work showed that in addition to the conserved coronavirus double membrane vesicles (DMVs), Beau-R, an apathogenic strain of avian Gammacoronavirus infectious bronchitis virus (IBV), induces regions of ER that are zippered together and tethered open-necked double membrane spherules that resemble replication organelles induced by other +RNA viruses. Here we compared structures induced by Beau-R with the pathogenic lab strain M41 to determine whether membrane rearrangements are strain dependent. Interestingly, M41 was found to have a low spherule phenotype. We then compared a panel of pathogenic, mild and attenuated IBV strains in ex vivo tracheal organ culture (TOC). Although the low spherule phenotype of M41 was conserved in TOCs, each of the other tested IBV strains produced DMVs, zippered ER and spherules. Furthermore, there was a significant correlation for the presence of DMVs with spherules, suggesting that these structures are spatially and temporally linked. Our data indicate that virus induced membrane rearrangements are fundamentally linked to the viral replicative machinery. However, coronavirus replicative apparatus clearly has the plasticity to function in different structural contexts.
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