Evidence map›Paper›PMID 27255409›Full record

ArticleImmunogenetics2016

Identification of interleukin genes in Pogona vitticeps using a de novo transcriptome assembly from RNA-seq data.

Alexandra Livernois, Kristine Hardy, Renae Domaschenz, Alexie Papanicolaou, Arthur Georges, Stephen D Sarre, Sudha Rao, Tariq Ezaz, Janine E Deakin

Abstract read
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In one paragraph

Article in Immunogenetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Alexandra LivernoisInstitute for Applied Ecology, University of Canberra, Canberra, ACT, 2617, Australia.
Kristine HardyDiscipline of Biomedical Sciences, Faculty of Education, Science, Technology and Mathematics, University of Canberra, Canberra, ACT, 2617, Australia.
Renae DomaschenzJohn Curtin School of Medical Research, Australian National University, Canberra, ACT, 2601, Australia.
Alexie PapanicolaouThe Hawkesbury Institute for the Environment, University of Western Sydney, Penrith, NSW, 2751, Australia.
Arthur GeorgesInstitute for Applied Ecology, University of Canberra, Canberra, ACT, 2617, Australia.
Stephen D SarreInstitute for Applied Ecology, University of Canberra, Canberra, ACT, 2617, Australia.
Sudha RaoDiscipline of Biomedical Sciences, Faculty of Education, Science, Technology and Mathematics, University of Canberra, Canberra, ACT, 2617, Australia.
Tariq EzazInstitute for Applied Ecology, University of Canberra, Canberra, ACT, 2617, Australia.
Janine E DeakinInstitute for Applied Ecology, University of Canberra, Canberra, ACT, 2617, Australia. janine.deakin@canberra.edu.au.
University of Canberra · AUAustralian National University · AUWestern Sydney University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Interleukins are a group of cytokines with complex immunomodulatory functions that are important for regulating immunity in vertebrate species. Reptiles and mammals last shared a common ancestor more than 350 million years ago, so it is not surprising that low sequence identity has prevented divergent interleukin genes from being identified in the central bearded dragon lizard, Pogona vitticeps, in its genome assembly. To determine the complete nucleotide sequences of key interleukin genes, we constructed full-length transcripts, using the Trinity platform, from short paired-end read RNA sequences from stimulated spleen cells. De novo transcript reconstruction and analysis allowed us to identify interleukin genes that are missing from the published P. vitticeps assembly. Identification of key cytokines in P. vitticeps will provide insight into the essential molecular mechanisms and evolution of interleukin gene families and allow for characterization of the immune response in a lizard for comparison with mammals.

Indexed as

Amino Acid SequenceAnimalsComputational BiologyGene Expression ProfilingHigh-Throughput Nucleotide SequencingInterleukinsLizardsSequence Analysis, RNASequence Homology, Amino AcidSoftwareTranscriptomeInterleukinsCytokineDe novo assemblyInterleukinPogona vitticeps

Identifiers

PMID27255409
OpenAlexW2419439740

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.