ArticleBMC developmental biology2016
Adhesive organ regeneration in Macrostomum lignano.
Article in BMC developmental biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Glycans in adhesion and fertilization: histochemical and ultrastructural insights from the chaetognath Spadella cephaloptera.Frontiers in zoology · 2026Article
- Amyloid-like Structures in Marine Adhesive Proteins.Marine drugs · 2025Review
- One probe fits all: a highly customizable modular RNA in situ hybridization platform expanding the application of SABER DNA probes.Development (Cambridge, England) · 2025Article
- A genetic and microscopy toolkit for manipulating and monitoring regeneration in Macrostomum lignano.Cell reports · 2024Article
- Optimized protocols for RNA interference in Macrostomum lignano.G3 (Bethesda, Md.) · 2024Article
- The muscle and neural architecture ofFrontiers in cellular and infection microbiology · 2024Article
- Glycoproteins Involved in Sea Urchin Temporary Adhesion.Marine drugs · 2023Article
- The Involvement of Cell-Type-Specific Glycans inBiomimetics (Basel, Switzerland) · 2022Article
- Sticking Together an Updated Model for Temporary Adhesion.Marine drugs · 2022Article
- (Un)expected Similarity of the Temporary Adhesive Systems of Marine, Brackish, and Freshwater Flatworms.International journal of molecular sciences · 2021Article
- RNA-Seq of three free-living flatworm species suggests rapid evolution of reproduction-related genes.BMC genomics · 2020Article
- Is the initiation of selfing linked to a hermaphrodite's female or male reproductive function?Behavioral ecology and sociobiology · 2020Article
- Temporary adhesion of the proseriate flatworm Minona ileanae.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Identifying adhesive components in a model tunicate.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Transcriptional characterisation of the Exaiptasia pallida pedal disc.BMC genomics · 2019Article
- A mechanism for temporary bioadhesion.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- The regenerative flatworm Macrostomum lignano, a model organism with high experimental potential.The International journal of developmental biology · 2018Review
- The structural and chemical basis of temporary adhesion in the sea starBeilstein journal of nanotechnology · 2018Article
- Profiling of adhesive-related genes in the freshwater cnidarian Hydra magnipapillata by transcriptomics and proteomics.Biofouling · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundFlatworms possess pluripotent stem cells that can give rise to all cell types, which allows them to restore lost body parts after injury or amputation. This makes flatworms excellent model systems for studying regeneration. In this study, we present the adhesive organs of a marine flatworm as a simple model system for organ regeneration. Macrostomum lignano has approximately 130 adhesive organs at the ventral side of its tail plate. One adhesive organ consists of three interacting cells: one adhesive gland cell, one releasing gland cell, and one modified epidermal cell, called an anchor cell. However, no specific markers for these cell types were available to study the regeneration of adhesive organs.
resultsWe tested 15 commercially available lectins for their ability to label adhesive organs and found one lectin (peanut agglutinin) to be specific to adhesive gland cells. We visualized the morphology of regenerating adhesive organs using lectin- and antibody staining as well as transmission electron microscopy. Our findings indicate that the two gland cells differentiate earlier than the connected anchor cells. Using EdU/lectin staining of partially amputated adhesive organs, we showed that their regeneration can proceed in two ways. First, adhesive gland cell bodies are able to survive partial amputation and reconnect with newly formed anchor cells. Second, adhesive gland cell bodies are cleared away, and the entire adhesive organ is build anew.
conclusionOur results provide the first insights into adhesive organ regeneration and describe ten new markers for differentiated cells and tissues in M. lignano. The position of adhesive organ cells within the blastema and their chronological differentiation have been shown for the first time. M. lignano can regenerate adhesive organs de novo but also replace individual anchor cells in an injured organ. Our findings contribute to a better understanding of organogenesis in flatworms and enable further molecular investigations of cell-fate decisions during regeneration.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.