ArticlePLoS genetics2016
Endogenous Mouse Dicer Is an Exclusively Cytoplasmic Protein.
Article in PLoS genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- An antisense-long-noncoding-RNA modulates p75iScience · 2025Article
- Long noncoding RNA Malat1 protects against osteoporosis and bone metastasis.Nature communications · 2024Article
- NANOS2 is a sequence-specific mRNA-binding protein that promotes transcript degradation in spermatogonial stem cells.iScience · 2021Article
- DROSHA is recruited to DNA damage sites by the MRN complex to promote non-homologous end joining.Journal of cell science · 2021Article
- DNA Damage-Induced Inflammatory Microenvironment and Adult Stem Cell Response.Frontiers in cell and developmental biology · 2021Review
- The nuclear DICER-circular RNA complex drives the deregulation of the glioblastoma cell microRNAome.Science advances · 2020Article
- Jack of all trades? The versatility of RNA in DNA double-strand break repair.Essays in biochemistry · 2020Review
- DICER regulates the expression of major satellite repeat transcripts and meiotic chromosome segregation during spermatogenesis.Nucleic acids research · 2020Article
- Alcohol induces mitochondrial fragmentation and stress responses to maintain normal muscle function in Caenorhabditis elegans.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Axonal precursor miRNAs hitchhike on endosomes and locally regulate the development of neural circuits.The EMBO journal · 2020Article
- Restricted and non-essential redundancy of RNAi and piRNA pathways in mouse oocytes.PLoS genetics · 2019Article
- Dgcr8 knockout approaches to understand microRNA functions in vitro and in vivo.Cellular and molecular life sciences : CMLS · 2019Review
- From "Cellular" RNA to "Smart" RNA: Multiple Roles of RNA in Genome Stability and Beyond.Chemical reviews · 2018Review
- Nuclear re-localization of Dicer in primary mouse embryonic fibroblast nuclei following DNA damage.PLoS genetics · 2018Article
- microRNAs Sculpt Neuronal Communication in a Tight Balance That Is Lost in Neurological Disease.Frontiers in molecular neuroscience · 2018Review
- Nuclear phosphorylated Dicer processes double-stranded RNA in response to DNA damage.The Journal of cell biology · 2017Article
- MicroRNA in Control of Gene Expression: An Overview of Nuclear Functions.International journal of molecular sciences · 2016Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
Dicer is a large multi-domain protein responsible for the ultimate step of microRNA and short-interfering RNA biogenesis. In human and mouse cell lines, Dicer has been shown to be important in the nuclear clearance of dsRNA as well as the establishment of chromatin modifications. Here we set out to unambiguously define the cellular localization of Dicer in mice to understand if this is a conserved feature of mammalian Dicer in vivo. To this end, we utilized an endogenously epitope tagged Dicer knock-in mouse allele. From primary mouse cell lines and adult tissues, we determined with certainty by biochemical fractionation and confocal immunofluorescence microscopy that endogenous Dicer is exclusively cytoplasmic. We ruled out the possibility that a fraction of Dicer shuttles to and from the nucleus as well as that FGF or DNA damage signaling induce Dicer nuclear translocation. We also explored Dicer localization during the dynamic and developmental context of embryogenesis, where Dicer is ubiquitously expressed and strictly cytoplasmic in all three germ layers as well as extraembryonic tissues. Our data exclude a direct role for Dicer in the nuclear RNA processing in the mouse.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.