ArticlePLoS pathogens2016
Small RNA-Based Antiviral Defense in the Phytopathogenic Fungus Colletotrichum higginsianum.
Article in PLoS pathogens, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 102 citations in OpenAlex.
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- Tracking of Tobacco Mosaic Virus in Taxonomically Different Plant Fungi.Journal of fungi (Basel, Switzerland) · 2025Article
- Molecular characterization of a novel non-segmented double stranded RNA mycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea.Archives of virology · 2025Article
- Fungal Argonaute proteins act in bidirectional cross-kingdom RNA interference during plant infection.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Reexamining the Mycovirome ofViruses · 2024Article
- Co-infection of Four Novel Mycoviruses from Three Lineages Confers Hypovirulence on Phytopathogenic Fungus Ustilaginoidea virens.Rice (New York, N.Y.) · 2024Article
- Argonaute-independent, Dicer-dependent antiviral defense against RNA viruses.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A Novel Strain of Fusarium oxysporum Virus 1 Isolated fromJournal of fungi (Basel, Switzerland) · 2024Article
- Function and regulation of plant ARGONAUTE proteins in response to environmental challenges: a review.PeerJ · 2024Review
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- Characterization of a novel hypovirus isolated from the phytopathogenic fungus Colletotrichum camelliae.Archives of virology · 2023Article
- RNA interference ofFrontiers in microbiology · 2023Article
- Similar Characteristics of siRNAs of Plant Viruses Which Replicate in Plant and Fungal Hosts.Biology · 2022Article
- Hypovirulence ofViruses · 2022Article
- Molecular mechanisms underlying host-induced gene silencing.The Plant cell · 2022Review
- Fusarium Graminearum Virus-1 Strain FgV1-SD4 Infection Eliminates Mycotoxin Deoxynivalenol Synthesis byMicroorganisms · 2022Article
- Mycovirus-encoded suppressors of RNA silencing: Possible allies or enemies in the use of RNAi to control fungal disease in crops.Frontiers in fungal biology · 2022Review
- Meiotic Silencing in DothideomycetousFrontiers in fungal biology · 2022Article
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
Even though the fungal kingdom contains more than 3 million species, little is known about the biological roles of RNA silencing in fungi. The Colletotrichum genus comprises fungal species that are pathogenic for a wide range of crop species worldwide. To investigate the role of RNA silencing in the ascomycete fungus Colletotrichum higginsianum, knock-out mutants affecting genes for three RNA-dependent RNA polymerase (RDR), two Dicer-like (DCL), and two Argonaute (AGO) proteins were generated by targeted gene replacement. No effects were observed on vegetative growth for any mutant strain when grown on complex or minimal media. However, Δdcl1, Δdcl1Δdcl2 double mutant, and Δago1 strains showed severe defects in conidiation and conidia morphology. Total RNA transcripts and small RNA populations were analyzed in parental and mutant strains. The greatest effects on both RNA populations was observed in the Δdcl1, Δdcl1Δdcl2, and Δago1 strains, in which a previously uncharacterized dsRNA mycovirus [termed Colletotrichum higginsianum non-segmented dsRNA virus 1 (ChNRV1)] was derepressed. Phylogenetic analyses clearly showed a close relationship between ChNRV1 and members of the segmented Partitiviridae family, despite the non-segmented nature of the genome. Immunoprecipitation of small RNAs associated with AGO1 showed abundant loading of 5'U-containing viral siRNA. C. higginsianum parental and Δdcl1 mutant strains cured of ChNRV1 revealed that the conidiation and spore morphology defects were primarily caused by ChNRV1. Based on these results, RNA silencing involving ChDCL1 and ChAGO1 in C. higginsianum is proposed to function as an antiviral mechanism.
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