ArticleEpigenetics & chromatin2016
Inheritance of the CENP-A chromatin domain is spatially and temporally constrained at human centromeres.
Article in Epigenetics & chromatin, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 42 citations in OpenAlex.
- The CENP-A chaperone complex spatially organizes centromeres.Science advances · 2026Article
- Neocentromeres fail to maintain DNA methylation boundaries, driving CENP-A drift, instability, and chromosome missegregation.Cell reports · 2026Article
- Epigenetic landscape of synthetic human centromere core regions: CENP-A assembly and euchromatic modifications interdependently antagonize heterochromatin accumulation.Nucleic acids research · 2026Article
- The CENP-A chaperone complex spatially organizes centromeres.bioRxiv : the preprint server for biology · 2025Article
- The dynamic centromere.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- Spindle checkpoint can secure additional cheating time for selfish expanded centromeres.Current biology : CB · 2025Article
- Epigenetic inheritance and boundary maintenance at human centromeres.Current opinion in structural biology · 2023Review
- CENP-I directly targets centromeric DNA to support CENP-A deposition and centromere maintenance.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- CENP-A: A Histone H3 Variant with Key Roles in Centromere Architecture in Healthy and Diseased States.Results and problems in cell differentiation · 2022Article
- Centromere Identity and the Regulation of Chromosome Segregation.Frontiers in cell and developmental biology · 2022Review
- Diverse mechanisms of centromere specification.Current biology : CB · 2021Review
- A genetic memory initiates the epigenetic loop necessary to preserve centromere position.The EMBO journal · 2020Article
- Genomic and functional variation of human centromeres.Experimental cell research · 2020Review
- CENP-C unwraps the human CENP-A nucleosome through the H2A C-terminal tail.EMBO reports · 2019Article
- Mechanism of centromere recruitment of the CENP-A chaperone HJURP and its implications for centromere licensing.Nature communications · 2019Article
- Centromeric and ectopic assembly of CENP-A chromatin in health and cancer: old marks and new tracks.Nucleic acids research · 2019Review
- Going the distance: Neocentromeres make long-range contacts with heterochromatin.The Journal of cell biology · 2019Article
- High-resolution mapping of centromeric protein association using APEX-chromatin fibers.Epigenetics & chromatin · 2018Article
- Inheritance of CENP-A Nucleosomes during DNA Replication Requires HJURP.Developmental cell · 2018Article
- Alpha satellite DNA biology: finding function in the recesses of the genome.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2018Review
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundChromatin containing the histone variant CENP-A (CEN chromatin) exists as an essential domain at every centromere and heritably marks the location of kinetochore assembly. The size of the CEN chromatin domain on alpha satellite DNA in humans has been shown to vary according to underlying array size. However, the average amount of CENP-A reported at human centromeres is largely consistent, implying the genomic extent of CENP-A chromatin domains more likely reflects variations in the number of CENP-A subdomains and/or the density of CENP-A nucleosomes within individual subdomains. Defining the organizational and spatial properties of CEN chromatin would provide insight into centromere inheritance via CENP-A loading in G1 and the dynamics of its distribution between mother and daughter strands during replication.
resultsUsing a multi-color protein strategy to detect distinct pools of CENP-A over several cell cycles, we show that nascent CENP-A is equally distributed to sister centromeres. CENP-A distribution is independent of previous or subsequent cell cycles in that centromeres showing disproportionately distributed CENP-A in one cycle can equally divide CENP-A nucleosomes in the next cycle. Furthermore, we show using extended chromatin fibers that maintenance of the CENP-A chromatin domain is achieved by a cycle-specific oscillating pattern of new CENP-A nucleosomes next to existing CENP-A nucleosomes over multiple cell cycles. Finally, we demonstrate that the size of the CENP-A domain does not change throughout the cell cycle and is spatially fixed to a similar location within a given alpha satellite DNA array.
conclusionsWe demonstrate that most human chromosomes share similar patterns of CENP-A loading and distribution and that centromere inheritance is achieved through specific placement of new CENP-A near existing CENP-A as assembly occurs each cell cycle. The loading pattern fixes the location and size of the CENP-A domain on individual chromosomes. These results suggest that spatial and temporal dynamics of CENP-A are important for maintaining centromere identity and genome stability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.