ArticleBiotechnology for biofuels2016
DNA microarray of global transcription factor mutant reveals membrane-related proteins involved in n-butanol tolerance in Escherichia coli.
Article in Biotechnology for biofuels, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 33 citations in OpenAlex.
- Systems-level analysis of 1,6-hexanediol stress response and tolerance engineering inSynthetic and systems biotechnology · 2027Article
- Advances in biosynthesis of higher alcohols in Escherichia coli.World journal of microbiology & biotechnology · 2023Review
- Current progress on engineering microbial strains and consortia for production of cellulosic butanol through consolidated bioprocessing.Microbial biotechnology · 2023Review
- Metabolic Engineering ofMicroorganisms · 2023Article
- Exposure to 1-Butanol Exemplifies the Response of the Thermoacidophilic Archaeon Sulfolobus acidocaldarius to Solvent Stress.Applied and environmental microbiology · 2021Article
- Butanol Tolerance ofGenes · 2021Article
- Increasing Solvent Tolerance to Improve Microbial Production of Alcohols, Terpenoids and Aromatics.Microorganisms · 2021Review
- Enhancing n-Butanol Tolerance of Escherichia coli by Overexpressing of Stress-Responsive Molecular Chaperones.Applied biochemistry and biotechnology · 2021Article
- Review
- Article
- Rapid Enabling ofFrontiers in bioengineering and biotechnology · 2021Article
- Propionic Acid Promotes the Virulent Phenotype of Crohn's Disease-Associated Adherent-Invasive Escherichia coli.Cell reports · 2020Article
- A Novel Butanol Tolerance-Promoting Function of the Transcription Factor Rob inFrontiers in bioengineering and biotechnology · 2020Article
- Physiological, Genetic, and Transcriptomic Analysis of Alcohol-Induced Delay ofApplied and environmental microbiology · 2019Article
- Enhancing butanol tolerance ofBiotechnology for biofuels · 2019Article
- Identification of functional butanol-tolerant genes fromBiotechnology for biofuels · 2019Article
- Understanding and engineering alcohol-tolerant bacteria using OMICS technology.World journal of microbiology & biotechnology · 2018Review
- Identification and manipulation of a novel locus to improve cell tolerance to short-chain alcohols in Escherichia coli.Journal of industrial microbiology & biotechnology · 2018Article
- Engineering global transcription to tune lipophilic properties inBiotechnology for biofuels · 2018Article
- Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production inBiotechnology for biofuels · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEscherichia coli has been explored as a platform host strain for biofuels production such as butanol. However, the severe toxicity of butanol is considered to be one major limitation for butanol production from E. coli. The goal of this study is therefore to construct butanol-tolerant E. coli strains and clarify the tolerance mechanisms.
resultsA recombinant E. coli strain harboring σ(70) mutation capable of tolerating 2 % (v/v) butanol was isolated by the global transcription machinery engineering (gTME) approach. DNA microarrays were employed to assess the transcriptome profile of butanol-tolerant strain B8. Compared with the wild-type strain, 329 differentially expressed genes (197 up-regulated and 132 down-regulated) (p < 0.05; FC ≥ 2) were identified. These genes are involved in carbohydrate metabolism, energy metabolism, two-component signal transduction system, oxidative stress response, lipid and cell envelope biogenesis and efflux pump.
conclusionsSeveral membrane-related proteins were proved to be involved in butanol tolerance of E. coli. Two down-regulated genes, yibT and yghW, were identified to be capable of affecting butanol tolerance by regulating membrane fatty acid composition. Another down-regulated gene ybjC encodes a predicted inner membrane protein. In addition, a number of up-regulated genes, such as gcl and glcF, contribute to supplement metabolic intermediates for glyoxylate and TCA cycles to enhance energy supply. Our results could serve as a practical strategy for the construction of platform E. coli strains as biofuel producer.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.