ArticleFrontiers in plant science2016
Cloning, Functional Characterization, and Catalytic Mechanism of a Bergaptol O-Methyltransferase from Peucedanum praeruptorum Dunn.
Article in Frontiers in plant science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- The role of coumarin biosynthesis in modulating plant drought responses: insights from PpBMT overexpression in Arabidopsis.BMC plant biology · 2026Article
- Integrated Metabolome and Transcriptome Analysis in Ammi majus L. Revealed Candidate Genes of Furanocoumarins Pathway.Biochemical genetics · 2026Article
- Quality formation inFrontiers in plant science · 2026Review
- The biosynthetic pathway of coumarin and its genetic regulation in response to biotic and abiotic stresses.Frontiers in plant science · 2025Review
- Article
- Two types of coumarins-specific enzymes complete the last missing steps in pyran- and furanocoumarins biosynthesis.Acta pharmaceutica Sinica. B · 2024Article
- Genome sequencing-based transcriptomic analysis reveals novel genes in Peucedanum praeruptorum.BMC genomic data · 2023Article
- Regioselective stilbene O-methylations in Saccharinae grasses.Nature communications · 2023Article
- Functional Diversification and Structural Origins of Plant Natural Product Methyltransferases.Molecules (Basel, Switzerland) · 2022Review
- Comparative Transcriptomics Unveil the Crucial Genes Involved in Coumarin Biosynthesis inFrontiers in plant science · 2022Article
- Article
- Crystal structure ofJournal of advanced research · 2020Article
- Structure-based tailoring of the first coumarins-specific bergaptol O-methyltransferase to synthesize bergapten for depigmentation disorder treatment.Journal of advanced research · 2020Article
- Convergent evolution of the UbiA prenyltransferase family underlies the independent acquisition of furanocoumarins in plants.The New phytologist · 2020Article
- 3'O-Methyltransferase, Ps3'OMT, from opium poppy: involvement in papaverine biosynthesis.Plant cell reports · 2019Article
- The Molecular and Structural Basis ofInternational journal of molecular sciences · 2019Article
- Elucidation of the biosynthesis pathway and heterologous construction of a sustainable route for producing umbelliferone.Journal of biological engineering · 2019Article
- Identification and functional characterization of a p-coumaroyl CoA 2'-hydroxylase involved in the biosynthesis of coumarin skeleton from Peucedanum praeruptorum Dunn.Plant molecular biology · 2017Article
- Cloning, Functional Characterization and Site-Directed Mutagenesis of 4-Coumarate: Coenzyme A Ligase (4CL) Involved in Coumarin Biosynthesis inFrontiers in plant science · 2017Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coumarins are main active components of Peucedanum praeruptorum Dunn. Among them, methoxylated coumarin compound, such as bergapten, xanthotoxin, and isopimpinellin, has high officinal value and plays an important role in medicinal field. However, major issues associated with the biosynthesis mechanism of coumarins remain unsolved and no corresponding enzyme has been cloned from P. praeruptorum. In this study, a local BLASTN program was conducted to find the candidate genes from P. praeruptorum transcriptome database using the nucleotide sequence of Ammi majus bergaptol O-methyltransferase (AmBMT, GenBank accession No: AY443006) as a template. As a result, a 1335 bp full-length of cDNA sequence which contains an open reading frame of 1080 bp encoding a BMT polypeptide of 359 amino acids was obtained. The recombinant protein was functionally expressed in Escherichia coli and displayed an observed activity to bergaptol. In vitro experiments show that the protein has narrow substrate specificity for bergaptol. Expression profile indicated that the cloned gene had a higher expression level in roots and can be induced by methyl jasmonate (MeJA). Subcellular localization analysis showed that the BMT protein was located in cytoplasm in planta. Homology modeling and docking based site-directed mutagenesis have been employed to investigate the amino acid residues in BMT required for substrate binding and catalysis. Conservative amino acid substitutions at residue H264 affected BMT catalysis, whereas substitutions at residues F171, M175, D226, and L312 affected substrate binding. The systemic study summarized here will enlarge our knowledge on OMTs and provide useful information in investigating the coumarins biosynthesis mechanism in P. praeruptorum.
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