ArticleJournal of experimental botany2016
Biofortification of rice with the essential amino acid lysine: molecular characterization, nutritional evaluation, and field performance.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 130 citations in OpenAlex.
- Exploring the functional food potential of wild rice (Food chemistry: X · 2026Article
- Moderate Regulation of SBEIIb Gene Expression by Editing Cis-Regulatory Elements Enhances the Resistant Starch Content in Rice.Plant biotechnology journal · 2026Article
- Genome-wide identification of theFrontiers in plant science · 2026Article
- Lysine-rich rice enhanced muscle growth and development in young rats.European journal of nutrition · 2025Article
- Navigating the challenges of engineering composite specialized metabolite pathways in plants.The Plant journal : for cell and molecular biology · 2025Review
- Review
- New Insights into the Genetic Basis of Lysine Accumulation in Rice Revealed by Multi-Model GWAS.International journal of molecular sciences · 2024Article
- Differences in cooking taste and physicochemical properties between compound nutritional rice and common rice.Frontiers in nutrition · 2024Article
- Identification of Candidate Genes that Affect the Contents of 17 Amino Acids in the Rice Grain Using a Genome-Wide Haplotype Association Study.Rice (New York, N.Y.) · 2023Article
- Genetically Modified Rice Is Associated with Hunger, Health, and Climate Resilience.Foods (Basel, Switzerland) · 2023Review
- Combined Hybridization and Evaluation of High-Lysine Rice: Nutritional and Physicochemical Qualities and Field Performance.International journal of molecular sciences · 2022Article
- Amino Acids in Rice Grains and Their Regulation by Polyamines and Phytohormones.Plants (Basel, Switzerland) · 2022Review
- CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement.International journal of molecular sciences · 2022Review
- Genes and Their Molecular Functions Determining Seed Structure, Components, and Quality of Rice.Rice (New York, N.Y.) · 2022Review
- Applications of Genomic Tools in Plant Breeding: Crop Biofortification.International journal of molecular sciences · 2022Review
- Extreme Low-Temperature Stress Affects Nutritional Quality of Amino Acids in Rice.Frontiers in plant science · 2022Article
- Advances in Crop Breeding Through Precision Genome Editing.Frontiers in genetics · 2022Review
- How do rice seedlings of landrace Pokkali survive in saline fields after transplantation? Physiology, biochemistry, and photosynthesis.Photosynthesis research · 2021Article
- Comparative Study of Physicochemical Properties and Starch Granule Structure in Seven Ginkgo Kernel Flours.Foods (Basel, Switzerland) · 2021Article
- Lysine biofortification in rice by modulating feedback inhibition of aspartate kinase and dihydrodipicolinate synthase.Plant biotechnology journal · 2021Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rice (Oryza sativa L.), a major staple crop worldwide, has limited levels of the essential amino acid lysine. We previously produced engineered rice with increased lysine content by expressing bacterial aspartate kinase and dihydrodipicolinate synthase and inhibiting rice lysine ketoglutarate reductase/saccharopine dehydrogenase activity. However, the grain quality, field performance, and integration patterns of the transgenes in these lysine-enriched lines remain unclear. In the present study, we selected several elite transgenic lines with endosperm-specific or constitutive regulation of the above key enzymes but lacking the selectable marker gene. All target transgenes were integrated into the intragenic region in the rice genome. Two pyramid transgenic lines (High Free Lysine; HFL1 and HFL2) with free lysine levels in seeds up to 25-fold that of wild type were obtained via a combination of the above two transgenic events. We observed a dramatic increase in total free amino acids and a slight increase in total protein content in both pyramid lines. Moreover, the general physicochemical properties were improved in pyramid transgenic rice, but the starch composition was not affected. Field trials indicated that the growth of HFL transgenic rice was normal, except for a slight difference in plant height and grain colour. Taken together, these findings will be useful for the potential commercialization of high-lysine transgenic rice.
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