Evidence map›Paper›PMID 27251294›Full record

ArticleParasites & vectors2016

Cryptosporidium and Giardia taxa in faecal samples from animals in catchments supplying the city of Melbourne with drinking water (2011 to 2015).

Anson V Koehler, Shane R Haydon, Aaron R Jex, Robin B Gasser

Abstract read
In one paragraph

Article in Parasites & vectors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed, 3 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed, 3 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
  3. Pooled it
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  7. Advancing genomics for waterborne pathogen surveillance in Australia.The Lancet regional health. Western Pacific · 2026
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  10. First record ofInternational journal for parasitology. Parasites and wildlife · 2024
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  11. Marked genetic diversity withinInternational journal for parasitology. Parasites and wildlife · 2024
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  12. New records ofInternational journal for parasitology. Parasites and wildlife · 2024
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  17. Molecular Detection ofVeterinary sciences · 2022
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  19. Multilocus Genotyping ofFrontiers in cellular and infection microbiology · 2022
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  20. An Update on ZoonoticAnimals : an open access journal from MDPI · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Anson V KoehlerFaculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria, 3010, Australia. anson.koehler@unimelb.edu.au.
Shane R HaydonMelbourne Water, Docklands, Victoria, 3001, Australia.
Aaron R JexFaculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria, 3010, Australia.
Robin B GasserFaculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria, 3010, Australia. robinbg@unimelb.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIn a long-term program to monitor pathogens in water catchments serving the City of Melbourne in the State of Victoria in Australia, we detected and genetically characterised Cryptosporidium and Giardia in faecal samples from various animals in nine water reservoir areas over a period of 4 years (July 2011 to November 2015).

methodsThis work was conducted using PCR-based single-strand conformation polymorphism (SSCP) and phylogenetic analyses of portions of the small subunit of ribosomal RNA (SSU) and 60 kDa glycoprotein (gp60) genes for Cryptosporidium, and triose-phosphate isomerase (tpi) gene for Giardia.

resultsThe prevalence of Cryptosporidium was 1.62 % (69 of 4,256 samples); 25 distinct sequence types were defined for pSSU, and six for gp60 which represented C. hominis (genotype Ib - subgenotype IbA10G2), C. cuniculus (genotype Vb - subgenotypes VbA26, and VbA25), and C. canis, C. fayeri, C. macropodum, C. parvum, C. ryanae, Cryptosporidium sp. "duck" genotype, C. suis and C. ubiquitum as well as 12 novel SSU sequence types. The prevalence of Giardia was 0.31 % (13 of 4,256 samples); all three distinct tpi sequence types defined represented assemblage A of G. duodenalis.

conclusionsOf the 34 sequence types (genotypes) characterized here, five and one have been recorded previously for Cryptosporidium and Giardia, respectively, from humans. Novel genotypes of Cryptosporidium and Giardia were recorded for SSU (n = 12), gp60 (n = 4) and tpi (n = 1); the zoonotic potential of these novel genotypes is presently unknown. Future work will continue to monitor the prevalence of Cryptosporidium and Giardia genotypes in animals in these catchments, and expand investigations to humans. Nucleotide sequences reported in this paper are available in the GenBank database under accession nos. KU531647-KU531718.

Indexed as

AnimalsCryptosporidiosisCryptosporidiumDNA, ProtozoanDNA, RibosomalDrinking WaterFecesGenotypeGeographyGiardiaGiardiasisHumansPhylogenyPolymorphism, Single-Stranded ConformationalProtozoan ProteinsRabbitsDNA, ProtozoanDNA, RibosomalDrinking WaterProtozoan Proteins60 kDa glycoprotein (gp60) geneCryptosporidiumGiardiaSingle-strand conformation polymorphism (SSCP)Small subunit (SSU) of ribosomal RNA geneTriose-phosphate isomerase (tpi) gene

Identifiers

PMID27251294
PMCPMC4888428

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.