Evidence map›Paper›PMID 27251159›Full record

ArticleIn vitro cellular & developmental biology. Animal2016

Quantitative comparison of cancer and normal cell adhesion using organosilane monolayer templates: an experimental study on the anti-adhesion effect of green-tea catechins.

Rumi Sakamoto, Eisuke Kakinuma, Kentaro Masuda, Yuko Takeuchi, Kosaku Ito, Kentaro Iketaki, Takahisa Matsuzaki, Seiichiro Nakabayashi, Hiroshi Y Yoshikawa, Hideaki Yamamoto and 2 more

Abstract read
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In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.7field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Rumi SakamotoSchool of Fundamental Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku, Tokyo, 169-8555, Japan.
Eisuke KakinumaSchool of Fundamental Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku, Tokyo, 169-8555, Japan.
Kentaro MasudaSchool of Fundamental Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku, Tokyo, 169-8555, Japan.
Yuko TakeuchiSchool of Fundamental Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku, Tokyo, 169-8555, Japan.
Kosaku ItoDepertment of Chemistry, Saitama University, 225 Shimo-ohkubo, Sakura-ku, Saitama, 338-8570, Japan.
Kentaro IketakiDepertment of Chemistry, Saitama University, 225 Shimo-ohkubo, Sakura-ku, Saitama, 338-8570, Japan.
Takahisa MatsuzakiDepertment of Chemistry, Saitama University, 225 Shimo-ohkubo, Sakura-ku, Saitama, 338-8570, Japan.
Seiichiro NakabayashiDepertment of Chemistry, Saitama University, 225 Shimo-ohkubo, Sakura-ku, Saitama, 338-8570, Japan.
Hiroshi Y YoshikawaDepertment of Chemistry, Saitama University, 225 Shimo-ohkubo, Sakura-ku, Saitama, 338-8570, Japan.
Hideaki YamamotoResearch Organization for Nano & Life Innovation, Waseda University, 513 Waseda-tsurumakicho, Shinjuku, Tokyo, 162-0041, Japan.
Yuko SatoResearch Organization for Nano & Life Innovation, Waseda University, 513 Waseda-tsurumakicho, Shinjuku, Tokyo, 162-0041, Japan.
Takashi TaniiSchool of Fundamental Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku, Tokyo, 169-8555, Japan. tanii@waseda.jp.
Waseda University · JPSaitama University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The main constituent of green tea, (-)-Epigallocatechin-3-O-gallate (EGCG), is known to have cancer-specific chemopreventive effects. In the present work, we investigated how EGCG suppresses cell adhesion by comparing the adhesion of human pancreatic cancer cells (AsPC-1 and BxPC-3) and their counterpart, normal human embryonic pancreas-derived cells (1C3D3), in catechin-containing media using organosilane monolayer templates (OMTs). The purpose of this work is (1) to evaluate the quantitativeness in the measurement of cell adhesion with the OMT and (2) to show how green-tea catechins suppress cell adhesion in a cancer-specific manner. For the first purpose, the adhesion of cancer and normal cells was compared using the OMT. The cell adhesion in different type of catechins such as EGCG, (-)-Epicatechin-3-O-gallate (ECG) and (-)-Epicatechin (EC) was also evaluated. The measurements revealed that the anti-adhesion effect of green-tea catechins is cancer-specific, and the order is EGCG≫ECG>EC. The results agree well with the data reported to date, showing the quantitativeness of the new method. For the second purpose, the contact area of cells on the OMT was measured by reflection interference contrast microscopy. The cell-OMT contact area of cancer cells decreases with increasing EGCG concentration, whereas that of normal cells remains constant. The results reveal a twofold action of EGCG on cancer cell adhesion-suppressing cell attachment to a candidate adhesion site and decreasing the contact area of the cells-and validates the use of OMT as a tool for screening cancer cell adhesion.

Indexed as

CatechinCell AdhesionCell Line, TumorHumansPancreasPancreatic NeoplasmsTeaCatechinepigallocatechin gallateTeaAnti-adhesion effectCell adhesionGreen-tea catechinOrganosilane monolayer templateReflection interference contrast microscopy

Identifiers

PMID27251159
OpenAlexW2408998367

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.